Detailed information of TA system TA036012

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 835757..836591
• Replicon: chromosome
• Accession: NZ_AP022536
• Organism: Escherichia coli strain THO-008

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E0IX31
• Locus tag: JLF34_RS03935
• Protein ID: WP_000854689.1
• Coordinates: 835757..836134 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0IVP8
• Locus tag: JLF34_RS03940
• Protein ID: WP_001285599.1
• Coordinates: 836211..836591 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF34_RS03905	832151..832306	-	156	WP_000729638.1	hypothetical protein	-
JLF34_RS03910	832738..833671	-	934	Protein_767	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JLF34_RS03915	833664..834059	-	396	WP_000208381.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
JLF34_RS03920	834128..834973	-	846	WP_001446750.1	DUF4942 domain-containing protein	-
JLF34_RS03925	835058..835255	-	198	WP_000839250.1	DUF957 domain-containing protein	-
JLF34_RS03930	835272..835760	-	489	WP_000761698.1	hypothetical protein	-
JLF34_RS03935	835757..836134	-	378	WP_000854689.1	TA system toxin CbtA family protein	Toxin
JLF34_RS03940	836211..836591	-	381	WP_001285599.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF34_RS03945	836641..837285	-	645	WP_000086757.1	hypothetical protein	-
JLF34_RS03950	837304..837525	-	222	WP_000692315.1	DUF987 domain-containing protein	-
JLF34_RS03955	837588..838064	-	477	WP_001186761.1	RadC family protein	-
JLF34_RS03960	838170..839669	+	1500	WP_000174402.1	IS21-like element ISEc10 family transposase	-
JLF34_RS03965	839666..840421	+	756	WP_000065240.1	IS21-like element ISEc10 family helper ATPase IstB	-
JLF34_RS03970	840496..840969	-	474	WP_001538139.1	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	832151..832306	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14042.07 Da
• Isoelectric Point: 9.1510

>T36012 WP_000854689.1 NZ_AP022536:c836134-835757 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T36012 NZ_AP022536:c836134-835757 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13984.78 Da
• Isoelectric Point: 5.0823

>AT36012 WP_001285599.1 NZ_AP022536:c836591-836211 [Escherichia coli]
MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLLQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT36012 NZ_AP022536:c836591-836211 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGTTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG