Detailed information of TA system TA035874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2621915..2622554
• Replicon: chromosome
• Accession: NZ_AP022513
• Organism: Citrobacter portucalensis strain STN0717-72

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: JLF48_RS12520
• Protein ID: WP_200921558.1
• Coordinates: 2622357..2622554 (-)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JLF48_RS12515
• Protein ID: WP_200921600.1
• Coordinates: 2621915..2622331 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF48_RS12495	2616962..2617906	-	945	WP_126957589.1	ABC transporter permease	-
JLF48_RS12500	2617907..2618923	-	1017	WP_063940776.1	ABC transporter ATP-binding protein	-
JLF48_RS12505	2618940..2620085	-	1146	WP_008784842.1	ABC transporter substrate-binding protein	-
JLF48_RS12510	2620413..2621822	-	1410	WP_148361941.1	PLP-dependent aminotransferase family protein	-
JLF48_RS12515	2621915..2622331	-	417	WP_200921600.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JLF48_RS12520	2622357..2622554	-	198	WP_200921558.1	type II toxin-antitoxin system HicA family toxin	Toxin
JLF48_RS12525	2622819..2624222	+	1404	WP_164562763.1	cytochrome ubiquinol oxidase subunit I	-
JLF48_RS12530	2624222..2625232	+	1011	WP_200921557.1	cytochrome d ubiquinol oxidase subunit II	-
JLF48_RS12535	2625232..2625357	+	126	WP_003020230.1	DUF2474 domain-containing protein	-
JLF48_RS12540	2625547..2625777	+	231	WP_003020233.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-66)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7440.58 Da
• Isoelectric Point: 10.9523

>T35874 WP_200921558.1 NZ_AP022513:c2622554-2622357 [Citrobacter portucalensis]
VKQSEFRRWLESQGVDVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLGLQCSLPVQG

Nucleotide

• Length: 198 bp

>T35874 NZ_AP022513:c2622554-2622357 [Citrobacter portucalensis]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTCGATGTGACAAATGGCTCAAATCATCTGAAGTTGAG
GTATCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCCGGTGATGAAATAAAAGAGGCCTTGCGAAAAGCGATCATTAAGC
AGCTTGGTTTACAGTGTTCATTACCAGTACAAGGTTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15092.26 Da
• Isoelectric Point: 4.3529

>AT35874 WP_200921600.1 NZ_AP022513:c2622331-2621915 [Citrobacter portucalensis]
MRYPVNLIPAVEGGFVVSFPDIPEALTQGETRHEALQAAQGALVTAFEFYFDDNEPIPLPSAVGTEADYVEIPLSVASKV
LLLNAFLESNITQQELANRIGRPKQEITRLFDLNHATKIDAVQIAARALGKELSLTML

Nucleotide

• Length: 417 bp

>AT35874 NZ_AP022513:c2622331-2621915 [Citrobacter portucalensis]
ATGCGTTATCCCGTTAATCTAATACCCGCCGTAGAAGGTGGATTTGTGGTTTCTTTCCCGGACATTCCTGAAGCATTGAC
TCAGGGAGAAACTCGCCATGAGGCTTTGCAAGCTGCACAGGGTGCATTGGTTACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTACCTTCTGCAGTAGGTACTGAAGCTGACTATGTGGAAATACCACTTAGCGTTGCGTCTAAAGTT
CTGTTGTTAAATGCCTTTCTTGAATCCAATATCACCCAGCAGGAACTGGCAAACAGGATCGGCAGGCCTAAGCAGGAGAT
CACTCGCTTATTCGATTTAAATCACGCGACAAAAATCGATGCAGTGCAGATAGCTGCGCGCGCCTTAGGCAAAGAACTGT
CATTAACGATGTTGTAA