Detailed information of TA system TA035868

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 681868..682547
• Replicon: chromosome
• Accession: NZ_AP022513
• Organism: Citrobacter portucalensis strain STN0717-72

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A7I6XXA8
• Locus tag: JLF48_RS03335
• Protein ID: WP_039025449.1
• Coordinates: 682206..682547 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: JLF48_RS03330
• Protein ID: WP_200922882.1
• Coordinates: 681868..682185 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF48_RS03290	676906..677214	+	309	WP_185666326.1	monooxygenase	-
JLF48_RS03295	677351..678223	-	873	WP_200922887.1	alpha/beta hydrolase	-
JLF48_RS03300	678600..678839	+	240	WP_200922886.1	YceI family protein	-
JLF48_RS03310	680462..680653	+	192	Protein_649	DUF905 family protein	-
JLF48_RS03315	680667..681128	+	462	WP_200922885.1	antirestriction protein	-
JLF48_RS03320	681137..681619	+	483	WP_200922884.1	DNA repair protein RadC	-
JLF48_RS03325	681628..681849	+	222	WP_200922883.1	DUF987 domain-containing protein	-
JLF48_RS03330	681868..682185	+	318	WP_200922882.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF48_RS03335	682206..682547	+	342	WP_039025449.1	TA system toxin CbtA family protein	Toxin
JLF48_RS03345	682910..683416	+	507	WP_149281949.1	G/U mismatch-specific DNA glycosylase	-
JLF48_RS03350	683462..685309	-	1848	WP_003024699.1	RNA polymerase sigma factor RpoD	-
JLF48_RS03355	685669..687414	-	1746	WP_200921149.1	DNA primase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	656826..687414	30588

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12880.79 Da
• Isoelectric Point: 9.6548

>T35868 WP_039025449.1 NZ_AP022513:682206-682547 [Citrobacter portucalensis]
MNTLPATTQRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFSDETVIREHINAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNSVQ

Nucleotide

• Length: 342 bp

>T35868 NZ_AP022513:682206-682547 [Citrobacter portucalensis]
ATGAATACTTTACCTGCAACAACCCAGCGGGCGGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGCTGCTGAC
CCGACTGCTCGAAAAGCACTATGGTCTGACCCTCAACGACACCCCGTTCAGCGATGAAACGGTTATTCGGGAACATATTA
ATGCTGGTATTACACTGGCTGATGCAGTGAATTTTCTGGTGGAAAAATACGAACTGGTCCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCACCTTATCTTCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAATTCGGTACAGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11854.56 Da
• Isoelectric Point: 6.9368

>AT35868 WP_200922882.1 NZ_AP022513:681868-682185 [Citrobacter portucalensis]
MSNITWGLQRAIMPCLGARLVQEGNRLHYLADRASITGKFSDAECRKLDETFPHFIRQMESMLTTGEVSPQHAHCVILYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT35868 NZ_AP022513:681868-682185 [Citrobacter portucalensis]
ATGAGCAATATCACATGGGGCCTGCAGCGGGCTATCATGCCGTGCCTGGGAGCTCGTCTGGTGCAGGAGGGTAACCGACT
GCACTACCTAGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAAGTCAGCCCCCAACATGCCCACTGCGTCATCCTGTACCAC
AACGGTTTCACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A7I6XXA8