Detailed information of TA system TA035816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 506975..507491
• Replicon: chromosome
• Accession: NZ_AP022498
• Organism: Enterobacter kobei strain STN0717-53

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A7I7DTN4
• Locus tag: JLH17_RS02430
• Protein ID: WP_045268867.1
• Coordinates: 507207..507491 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8E1RL94
• Locus tag: JLH17_RS02425
• Protein ID: WP_023331889.1
• Coordinates: 506975..507217 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLH17_RS02410	503070..503813	+	744	WP_045268866.1	KDGP aldolase family protein	-
JLH17_RS02415	503831..504967	+	1137	WP_023338954.1	lactonase family protein	-
JLH17_RS02420	504987..506897	+	1911	WP_023338955.1	BglG family transcription antiterminator	-
JLH17_RS02425	506975..507217	+	243	WP_023331889.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
JLH17_RS02430	507207..507491	+	285	WP_045268867.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLH17_RS02435	507495..507959	-	465	WP_032637633.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
JLH17_RS02440	508101..510239	-	2139	WP_014882293.1	anaerobic ribonucleoside-triphosphate reductase	-
JLH17_RS02445	510609..511181	-	573	WP_023331891.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10936.77 Da
• Isoelectric Point: 10.1988

>T35816 WP_045268867.1 NZ_AP022498:507207-507491 [Enterobacter kobei]
MIYELVFDPRALKEWSKLGDTVKNQFKKKLADVLVNPRVESTRLHGLPDCYKIKLRSQGYRLVYQVQDDVVTVFVIAVGK
REKSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T35816 NZ_AP022498:507207-507491 [Enterobacter kobei]
ATGATTTATGAACTGGTATTTGATCCGAGGGCGTTAAAGGAGTGGAGCAAGCTGGGCGATACCGTAAAAAACCAATTTAA
GAAGAAGCTTGCGGACGTGTTGGTGAATCCCAGGGTTGAATCGACTCGCCTGCATGGCCTGCCTGATTGCTACAAGATAA
AACTGAGATCCCAGGGTTATCGTTTGGTCTATCAGGTTCAGGATGACGTTGTAACGGTATTTGTTATTGCTGTCGGTAAG
AGAGAGAAGTCAGCCGTTTATCATGACGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9030.41 Da
• Isoelectric Point: 4.7719

>AT35816 WP_023331889.1 NZ_AP022498:506975-507217 [Enterobacter kobei]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTISDSEDELIQTVRERLANPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT35816 NZ_AP022498:506975-507217 [Enterobacter kobei]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACGCC
AACGGAAGCTGTGCGTTTACTGTTTCAGTATGTCGCGGAAACGGGACGCATGCCGGTGAAAACGGTCACCATTAGCGACA
GTGAAGATGAACTGATACAGACAGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGAATTAAGGTCAGTCTGGATGATTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A7I7DTN4