Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2565178..2565740 Replicon chromosome
Accession NZ_AP022478
Organism Escherichia coli strain STN0717-11

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag JLF30_RS12050 Protein ID WP_000605676.1
Coordinates 2565178..2565456 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JLF30_RS12055 Protein ID WP_000781370.1
Coordinates 2565456..2565740 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF30_RS12025 2560764..2561195 - 432 WP_000152305.1 peroxiredoxin OsmC -
JLF30_RS12030 2561540..2561755 + 216 WP_000495766.1 biofilm-dependent modulation protein -
JLF30_RS12035 2561857..2561994 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JLF30_RS12040 2562151..2563848 + 1698 WP_000433464.1 malate dehydrogenase -
JLF30_RS12045 2563982..2564992 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
JLF30_RS12050 2565178..2565456 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JLF30_RS12055 2565456..2565740 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
JLF30_RS12060 2565968..2566621 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
JLF30_RS12065 2566614..2567498 - 885 WP_001240582.1 formate dehydrogenase N subunit beta -
JLF30_RS12070 2567511..2570558 - 3048 WP_077577597.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T35725 WP_000605676.1 NZ_AP022478:2565178-2565456 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T35725 NZ_AP022478:2565178-2565456 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT35725 WP_000781370.1 NZ_AP022478:2565456-2565740 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT35725 NZ_AP022478:2565456-2565740 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References