Detailed information of TA system TA035702

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3448875..3449539
• Replicon: chromosome
• Accession: NZ_AP022465
• Organism: Enterobacter roggenkampii strain STW0522-66

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A157QTP3
• Locus tag: JLF42_RS16505
• Protein ID: WP_007869731.1
• Coordinates: 3449213..3449539 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A0M7FI99
• Locus tag: JLF42_RS16500
• Protein ID: WP_007869729.1
• Coordinates: 3448875..3449192 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF42_RS16460	3444954..3445691	+	738	WP_044593432.1	DeoR family transcriptional regulator	-
JLF42_RS16465	3445748..3446020	+	273	WP_007869719.1	hypothetical protein	-
JLF42_RS16470	3446100..3446360	+	261	WP_058691045.1	hypothetical protein	-
JLF42_RS16475	3446487..3447308	+	822	WP_007869723.1	DUF945 domain-containing protein	-
JLF42_RS16480	3447332..3447571	+	240	WP_007869725.1	DUF905 domain-containing protein	-
JLF42_RS16485	3447675..3448133	+	459	WP_013098170.1	antirestriction protein	-
JLF42_RS16490	3448149..3448625	+	477	WP_013098171.1	RadC family protein	-
JLF42_RS16495	3448634..3448861	+	228	WP_063929804.1	DUF987 domain-containing protein	-
JLF42_RS16500	3448875..3449192	+	318	WP_007869729.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF42_RS16505	3449213..3449539	+	327	WP_007869731.1	TA system toxin CbtA family protein	Toxin
JLF42_RS16510	3449770..3451107	-	1338	WP_055321858.1	type VI secretion system ImpA and VasL domain-containing protein	-
JLF42_RS16515	3451154..3452398	-	1245	WP_031275389.1	GIY-YIG nuclease family protein	-
JLF42_RS16520	3453098..3454360	-	1263	Protein_3242	type VI secretion system contractile sheath large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3404629..3451107	46478

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-106)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12299.25 Da
• Isoelectric Point: 8.9951

>T35702 WP_007869731.1 NZ_AP022465:3449213-3449539 [Enterobacter roggenkampii]
MKTLPATTQRAAKPCLSPVAVWQMLLARLLEQHYGLTINDTPFSDEVVIQQHIDAGITLSDAVNFLVEKYELVRIDRKGF
RWQEQSPYLRAVDILRARQAIGLLRGSH

Nucleotide

• Length: 327 bp

>T35702 NZ_AP022465:3449213-3449539 [Enterobacter roggenkampii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCAGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGGC
GCGTTTGCTGGAACAGCACTACGGTCTGACAATAAACGACACCCCATTCAGCGATGAAGTTGTTATACAGCAACATATCG
ATGCCGGTATCACCCTGTCAGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTCCGTATCGACAGGAAGGGATTT
CGCTGGCAGGAACAATCCCCTTACCTTCGGGCTGTCGATATTCTGAGGGCAAGACAGGCAATAGGTCTTTTACGAGGATC
TCATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11917.60 Da
• Isoelectric Point: 7.0264

>AT35702 WP_007869729.1 NZ_AP022465:3448875-3449192 [Enterobacter roggenkampii]
MTHIIWGLQRAITPRLGARLVQEGNRLHYLADRASITGMFSDAECRKLDDTFPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT35702 NZ_AP022465:3448875-3449192 [Enterobacter roggenkampii]
ATGACCCACATCATCTGGGGCCTGCAGCGGGCTATCACGCCGCGCCTGGGAGCCCGTCTGGTACAGGAGGGCAACCGGCT
GCACTACCTGGCTGACCGGGCCAGCATCACCGGTATGTTCAGTGACGCCGAATGCCGGAAGCTGGATGACACATTCCCAC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCAACATGCCCACTGCGTCACCCTGTACCAC
AACGGTTTTACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A157QTP3

Antitoxin

• AlphaFold DB: A0A0M7FI99