Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 181046..181653 Replicon chromosome
Accession NZ_AP022465
Organism Enterobacter roggenkampii strain STW0522-66

Toxin (Protein)


Gene name hicA Uniprot ID A0A7H0N5B9
Locus tag JLF42_RS00875 Protein ID WP_071993467.1
Coordinates 181046..181231 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A155E9Y3
Locus tag JLF42_RS00880 Protein ID WP_032675741.1
Coordinates 181246..181653 (+) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF42_RS00860 177478..179016 + 1539 WP_023345218.1 aldehyde dehydrogenase AldB -
JLF42_RS00865 179013..179978 - 966 WP_200925735.1 LysR family transcriptional regulator -
JLF42_RS00870 180096..180836 + 741 WP_047748126.1 MipA/OmpV family protein -
JLF42_RS00875 181046..181231 + 186 WP_071993467.1 type II toxin-antitoxin system HicA family toxin Toxin
JLF42_RS00880 181246..181653 + 408 WP_032675741.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JLF42_RS00885 181657..182733 - 1077 WP_032654523.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
JLF42_RS00890 183079..183774 - 696 WP_063930159.1 L-ribulose-5-phosphate 4-epimerase -
JLF42_RS00895 183768..184628 - 861 WP_021242445.1 L-ribulose-5-phosphate 3-epimerase -
JLF42_RS00900 184631..185284 - 654 WP_063930158.1 3-keto-L-gulonate-6-phosphate decarboxylase UlaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6882.06 Da        Isoelectric Point: 11.2341

>T35687 WP_071993467.1 NZ_AP022465:181046-181231 [Enterobacter roggenkampii]
VKSADVITVLISHGWKCVRTKGSHHQFRHPEQKGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Download         Length: 186 bp

>T35687 NZ_AP022465:181046-181231 [Enterobacter roggenkampii]
GTGAAAAGTGCGGATGTCATTACTGTTCTGATCAGCCATGGCTGGAAATGTGTCAGAACGAAAGGAAGCCATCATCAGTT
TCGCCATCCCGAACAGAAAGGGCTGGTTACTGTCCCACATCCCAAGAAGGATATTAAACCGGGCACTCTCGCCCAGATCT
GGCGACAGGCGGGTATTAAACACTGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 15010.78 Da        Isoelectric Point: 4.3897

>AT35687 WP_032675741.1 NZ_AP022465:181246-181653 [Enterobacter roggenkampii]
MFYPAYIHSDSDGSASGFFPDVPGCFFAGDSLDDAFQDARDALTAHFEALFEMDNALPLPGNVEAHLESRPEDFTGGQWL
LVDINMKQFDGRAERINITMPRRLLVKIDSFVSEHPQFGNRSAFLAEAARRVLPG

Download         Length: 408 bp

>AT35687 NZ_AP022465:181246-181653 [Enterobacter roggenkampii]
ATGTTTTATCCGGCTTACATTCATTCTGACTCCGACGGTAGCGCCAGCGGCTTTTTCCCTGACGTTCCAGGCTGTTTTTT
TGCAGGTGATTCCCTGGACGACGCTTTTCAGGATGCGCGTGATGCGCTCACAGCACACTTCGAAGCGCTATTTGAAATGG
ATAATGCACTGCCTCTTCCGGGTAATGTGGAAGCGCATCTGGAAAGTCGTCCTGAGGATTTCACTGGCGGGCAATGGCTG
TTGGTAGATATTAATATGAAACAGTTCGACGGTAGGGCCGAGCGTATCAACATCACGATGCCTCGACGTTTGCTGGTAAA
AATTGATTCCTTTGTCAGCGAGCATCCTCAGTTCGGTAATCGAAGCGCATTTCTGGCTGAGGCAGCACGTCGCGTGCTGC
CTGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H0N5B9

Antitoxin

Source ID Structure
AlphaFold DB A0A155E9Y3

References