Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1161923..1162543 Replicon chromosome
Accession NZ_AP022394
Organism Citrobacter portucalensis strain STW0522-27

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag JLF33_RS05505 Protein ID WP_002892050.1
Coordinates 1161923..1162141 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag JLF33_RS05510 Protein ID WP_003021733.1
Coordinates 1162169..1162543 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF33_RS05470 1157281..1157910 + 630 WP_003831032.1 membrane protein -
JLF33_RS05475 1157975..1158328 + 354 WP_003831033.1 DUF1428 family protein -
JLF33_RS05480 1158380..1159933 - 1554 WP_200920716.1 EAL domain-containing protein -
JLF33_RS05485 1160122..1160382 + 261 WP_008783961.1 type B 50S ribosomal protein L31 -
JLF33_RS05490 1160384..1160524 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
JLF33_RS05495 1160603..1161073 - 471 WP_003021724.1 YlaC family protein -
JLF33_RS05500 1161190..1161741 - 552 WP_003835924.1 maltose O-acetyltransferase -
JLF33_RS05505 1161923..1162141 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
JLF33_RS05510 1162169..1162543 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
JLF33_RS05515 1163032..1166181 - 3150 WP_193135115.1 efflux RND transporter permease AcrB -
JLF33_RS05520 1166204..1167397 - 1194 WP_003831044.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T35556 WP_002892050.1 NZ_AP022394:c1162141-1161923 [Citrobacter portucalensis]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T35556 NZ_AP022394:c1162141-1161923 [Citrobacter portucalensis]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT35556 WP_003021733.1 NZ_AP022394:c1162543-1162169 [Citrobacter portucalensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT35556 NZ_AP022394:c1162543-1162169 [Citrobacter portucalensis]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTTAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References