Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3088344..3089175 Replicon chromosome
Accession NZ_AP022362
Organism Escherichia coli strain E302

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag G6M96_RS15465 Protein ID WP_000854815.1
Coordinates 3088801..3089175 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag G6M96_RS15460 Protein ID WP_001280918.1
Coordinates 3088344..3088712 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6M96_RS15415 3083435..3084179 + 745 Protein_3021 IS21-like element ISEc12 family helper ATPase IstB -
G6M96_RS15420 3084262..3084612 + 351 Protein_3022 hypothetical protein -
G6M96_RS15425 3084628..3085038 + 411 WP_000846703.1 hypothetical protein -
G6M96_RS15430 3085259..3086077 + 819 WP_001542275.1 DUF945 domain-containing protein -
G6M96_RS15435 3086077..3086322 + 246 WP_001164966.1 hypothetical protein -
G6M96_RS15440 3086416..3086889 + 474 WP_001542276.1 antirestriction protein -
G6M96_RS15445 3086905..3087381 + 477 WP_001186200.1 RadC family protein -
G6M96_RS15450 3087444..3087665 + 222 WP_000692345.1 DUF987 domain-containing protein -
G6M96_RS15455 3087684..3088328 + 645 WP_000086752.1 hypothetical protein -
G6M96_RS15460 3088344..3088712 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
G6M96_RS15465 3088801..3089175 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
G6M96_RS15470 3089172..3089366 + 195 WP_000988600.1 hypothetical protein -
G6M96_RS15475 3089379..3089492 + 114 WP_001161660.1 DUF957 domain-containing protein -
G6M96_RS15480 3089781..3089954 - 174 WP_032082634.1 transposase domain-containing protein -
G6M96_RS15485 3089981..3090163 + 183 WP_001296208.1 hypothetical protein -
G6M96_RS15490 3090264..3090593 - 330 WP_000450409.1 DUF496 family protein -
G6M96_RS15495 3090765..3091823 - 1059 WP_001200889.1 FUSC family protein -
G6M96_RS15500 3092021..3092494 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
G6M96_RS15505 3092613..3093779 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T35469 WP_000854815.1 NZ_AP022362:3088801-3089175 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T35469 NZ_AP022362:3088801-3089175 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT35469 WP_001280918.1 NZ_AP022362:3088344-3088712 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT35469 NZ_AP022362:3088344-3088712 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References