Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2448934..2449496 Replicon chromosome
Accession NZ_AP022298
Organism Escherichia coli strain BEC1-S17-ESBL-09

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag H7R48_RS11660 Protein ID WP_077557414.1
Coordinates 2449218..2449496 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag H7R48_RS11655 Protein ID WP_000781370.1
Coordinates 2448934..2449218 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R48_RS11640 2444116..2447163 + 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -
H7R48_RS11645 2447176..2448060 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
H7R48_RS11650 2448053..2448706 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
H7R48_RS11655 2448934..2449218 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
H7R48_RS11660 2449218..2449496 - 279 WP_077557414.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R48_RS11665 2449682..2450692 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
H7R48_RS11670 2450826..2452523 - 1698 WP_000433464.1 malate dehydrogenase -
H7R48_RS11675 2452680..2452817 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
H7R48_RS11680 2452919..2453134 - 216 WP_000495766.1 biofilm-dependent modulation protein -
H7R48_RS11685 2453479..2453910 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10506.09 Da        Isoelectric Point: 6.8675

>T35259 WP_077557414.1 NZ_AP022298:c2449496-2449218 [Escherichia coli]
MIMNFRHKGLRDLFLLGITSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T35259 NZ_AP022298:c2449496-2449218 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCATAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT35259 WP_000781370.1 NZ_AP022298:c2449218-2448934 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT35259 NZ_AP022298:c2449218-2448934 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References