Detailed information of TA system TA035188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 28546..29239
• Replicon: plasmid pWP8-W19-CRE-03_2
• Accession: NZ_AP022283
• Organism: Aeromonas veronii strain WP8-W19-CRE-03

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: H7R74_RS21985
• Protein ID: WP_000182276.1
• Coordinates: 28883..29239 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: H7R74_RS21980
• Protein ID: WP_001172026.1
• Coordinates: 28546..28881 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R74_RS21935	23825..24400	-	576	WP_001173919.1	recombinase family protein	-
H7R74_RS21940	24527..24778	-	252	WP_130627584.1	type II toxin-antitoxin system YafQ family toxin	-
H7R74_RS21945	24937..25176	+	240	WP_003089107.1	ribbon-helix-helix domain-containing protein	-
H7R74_RS21950	25176..25586	+	411	WP_003150546.1	putative toxin-antitoxin system toxin component, PIN family	-
H7R74_RS21955	25590..25910	+	321	Protein_32	DUF4158 domain-containing protein	-
H7R74_RS21960	26078..27351	-	1274	Protein_33	HD-GYP domain-containing protein	-
H7R74_RS21965	27469..27849	-	381	WP_001054412.1	hypothetical protein	-
H7R74_RS21970	27846..28172	-	327	WP_000091614.1	hypothetical protein	-
H7R74_RS21975	28196..28531	-	336	WP_000741275.1	hypothetical protein	-
H7R74_RS21980	28546..28881	-	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
H7R74_RS21985	28883..29239	-	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R74_RS21990	29431..30033	+	603	WP_010465829.1	recombinase family protein	-
H7R74_RS21995	30017..33046	+	3030	WP_032432545.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2	-	1..47395	47395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T35188 WP_000182276.1 NZ_AP022283:c29239-28883 [Aeromonas veronii]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T35188 NZ_AP022283:c29239-28883 [Aeromonas veronii]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGAGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGAAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT35188 WP_001172026.1 NZ_AP022283:c28881-28546 [Aeromonas veronii]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT35188 NZ_AP022283:c28881-28546 [Aeromonas veronii]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7