Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1255292..1255912 Replicon chromosome
Accession NZ_AP022126
Organism Enterobacter cloacae strain WP5-S18-CRE-02

Toxin (Protein)


Gene name Hha Uniprot ID A0A2W0G488
Locus tag H7R61_RS06060 Protein ID WP_014882802.1
Coordinates 1255292..1255510 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag H7R61_RS06065 Protein ID WP_008499288.1
Coordinates 1255538..1255912 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R61_RS06030 1251301..1251561 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
H7R61_RS06035 1251564..1251704 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
H7R61_RS06040 1251701..1252411 - 711 WP_014882798.1 GNAT family N-acetyltransferase -
H7R61_RS06045 1252513..1253973 + 1461 WP_014882799.1 PLP-dependent aminotransferase family protein -
H7R61_RS06050 1253945..1254412 - 468 WP_014882800.1 membrane protein -
H7R61_RS06055 1254531..1255082 - 552 WP_014882801.1 maltose O-acetyltransferase -
H7R61_RS06060 1255292..1255510 - 219 WP_014882802.1 hemolysin expression modulator Hha Toxin
H7R61_RS06065 1255538..1255912 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
H7R61_RS06070 1256424..1259570 - 3147 WP_014882803.1 multidrug efflux RND transporter permease subunit -
H7R61_RS06075 1259593..1260786 - 1194 WP_014882804.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T34658 WP_014882802.1 NZ_AP022126:c1255510-1255292 [Enterobacter cloacae]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T34658 NZ_AP022126:c1255510-1255292 [Enterobacter cloacae]
ATGTCTGAAAAACCACTCACAAAGGTCGATTATTTGATGCGCTTACGCCGTTGTCAGTCAATTGACACCCTCGAACGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGCCTCGCTGAGC
TAACGATGAATAAGTTATATGACAAAATTCCCACATCCGTGTGGAAATTTGTACGTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT34658 WP_008499288.1 NZ_AP022126:c1255912-1255538 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT34658 NZ_AP022126:c1255912-1255538 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAACGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGATCCAACGTCTGCCATCAATTTACAGTTGAACGAGCTTATAGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCGCAAGATTTGCAAAAATGGCGCAAATCGGGAAACCGACT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCCGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G488

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References