Detailed information of TA system TA034481

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53734..54155
• Replicon: plasmid pWP4-S18-ESBL-08_1
• Accession: NZ_AP022096
• Organism: Escherichia coli strain WP4-S18-ESBL-08

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: H7R08_RS23930
• Protein ID: WP_001302184.1
• Coordinates: 53997..54155 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	53734..53932 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R08_RS23900	48837..49346	-	510	WP_071600123.1	hypothetical protein	-
H7R08_RS23905	49664..50203	+	540	WP_000290801.1	single-stranded DNA-binding protein	-
H7R08_RS23910	50260..50493	+	234	WP_000005990.1	DUF905 family protein	-
H7R08_RS23915	50559..52517	+	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
H7R08_RS23920	52572..53006	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
H7R08_RS23925	53003..53722	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	53734..53932	+	199	NuclAT_0	-	Antitoxin
-	53734..53932	+	199	NuclAT_0	-	Antitoxin
-	53734..53932	+	199	NuclAT_0	-	Antitoxin
-	53734..53932	+	199	NuclAT_0	-	Antitoxin
H7R08_RS23930	53997..54155	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R08_RS23935	54393..54752	-	360	Protein_56	hypothetical protein	-
H7R08_RS23940	54822..55028	+	207	WP_000547965.1	hypothetical protein	-
H7R08_RS23945	55053..55340	+	288	WP_000107546.1	hypothetical protein	-
H7R08_RS23950	55459..56280	+	822	WP_001234475.1	DUF945 domain-containing protein	-
H7R08_RS23955	56575..57165	-	591	WP_166494936.1	transglycosylase SLT domain-containing protein	-
H7R08_RS23960	57517..57900	+	384	WP_001063020.1	relaxosome protein TraM	-
H7R08_RS23965	58092..58739	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
H7R08_RS23970	58875..59090	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaTEM-1B	iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB	1..158292	158292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T34481 WP_001302184.1 NZ_AP022096:53997-54155 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T34481 NZ_AP022096:53997-54155 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT34481 NZ_AP022096:53734-53932 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGACGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin