Detailed information of TA system TA034393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 55472..56019
• Replicon: plasmid pWP4-W18-ESBL-09_3
• Accession: NZ_AP022072
• Organism: Escherichia coli strain WP4-W18-ESBL-09

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: H7R28_RS26730
• Protein ID: WP_000970000.1
• Coordinates: 55738..56019 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: K4JYR0
• Locus tag: H7R28_RS26725
• Protein ID: WP_000079928.1
• Coordinates: 55472..55741 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R28_RS26685	50487..50948	+	462	WP_016230918.1	thermonuclease family protein	-
H7R28_RS26690	50994..51203	+	210	WP_001369435.1	hemolysin expression modulator Hha	-
H7R28_RS26695	51241..51831	+	591	WP_042018152.1	DUF2726 domain-containing protein	-
H7R28_RS26700	51986..52459	+	474	WP_016240489.1	hypothetical protein	-
-	52602..52661	-	60	NuclAT_1	-	-
-	52602..52661	-	60	NuclAT_1	-	-
-	52602..52661	-	60	NuclAT_1	-	-
-	52602..52661	-	60	NuclAT_1	-	-
H7R28_RS26705	52706..52855	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	-
H7R28_RS26710	53140..53388	+	249	WP_000083839.1	replication regulatory protein RepA	-
H7R28_RS26715	53633..53707	+	75	WP_001365705.1	RepA leader peptide Tap	-
H7R28_RS26720	53700..54557	+	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-
H7R28_RS26725	55472..55741	+	270	WP_000079928.1	hypothetical protein	Antitoxin
H7R28_RS26730	55738..56019	+	282	WP_000970000.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R28_RS26735	56065..56913	+	849	WP_032256585.1	3'-5' exonuclease	-
H7R28_RS26740	57030..57512	+	483	WP_001311056.1	hypothetical protein	-
H7R28_RS26745	57814..58161	+	348	WP_088251382.1	hypothetical protein	-
H7R28_RS26750	58179..58769	-	591	WP_032256264.1	hypothetical protein	-
H7R28_RS26755	58993..59178	+	186	WP_032256266.1	hypothetical protein	-
H7R28_RS26760	59241..60110	-	870	WP_032256267.1	hypothetical protein	-
H7R28_RS26765	60658..60876	+	219	WP_032256270.1	type II toxin-antitoxin system antitoxin CcdA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..77891	77891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10921.62 Da
• Isoelectric Point: 8.4615

>T34393 WP_000970000.1 NZ_AP022072:55738-56019 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLVGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T34393 NZ_AP022072:55738-56019 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGTCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCACATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10175.50 Da
• Isoelectric Point: 7.3210

>AT34393 WP_000079928.1 NZ_AP022072:55472-55741 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT34393 NZ_AP022072:55472-55741 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5W2J4D0