Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 66972..67699 Replicon plasmid pWP3-W18-ESBL-02_2
Accession NZ_AP021974
Organism Klebsiella sp. WP3-W18-ESBL-02

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag H7R56_RS26540 Protein ID WP_000558568.1
Coordinates 66972..67283 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag H7R56_RS26545 Protein ID WP_000990392.1
Coordinates 67280..67699 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R56_RS26520 64253..64510 - 258 WP_106931027.1 hypothetical protein -
H7R56_RS26525 64537..65364 - 828 WP_106931019.1 ParA family protein -
H7R56_RS26530 65511..66086 + 576 WP_106931017.1 hypothetical protein -
H7R56_RS26535 66120..66824 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
H7R56_RS26540 66972..67283 + 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
H7R56_RS26545 67280..67699 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
H7R56_RS26550 67736..67945 - 210 WP_020806042.1 hypothetical protein -
H7R56_RS26555 68864..69844 - 981 WP_033544729.1 IS5-like element ISKpn26 family transposase -
H7R56_RS26560 69913..72219 + 2307 Protein_81 poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 68864..69844 980
- inside Non-Mobilizable plasmid - - 1..146141 146141


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T34000 WP_000558568.1 NZ_AP021974:66972-67283 [Klebsiella sp. WP3-W18-ESBL-02]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T34000 NZ_AP021974:66972-67283 [Klebsiella sp. WP3-W18-ESBL-02]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT34000 WP_000990392.1 NZ_AP021974:67280-67699 [Klebsiella sp. WP3-W18-ESBL-02]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT34000 NZ_AP021974:67280-67699 [Klebsiella sp. WP3-W18-ESBL-02]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References