Detailed information of TA system TA033873

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 57623..57887
• Replicon: plasmid pWP2-S18-ESBL-07_1
• Accession: NZ_AP021945
• Organism: Escherichia coli strain WP2-S18-ESBL-07

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: H7R05_RS23020
• Protein ID: WP_001331364.1
• Coordinates: 57735..57887 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	57623..57680 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R05_RS23005	53961..55031	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
H7R05_RS23010	55050..56258	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
H7R05_RS23015	56476..57462	-	987	WP_001257838.1	hypothetical protein	-
-	57623..57680	-	58	NuclAT_0	-	Antitoxin
-	57623..57680	-	58	NuclAT_0	-	Antitoxin
-	57623..57680	-	58	NuclAT_0	-	Antitoxin
-	57623..57680	-	58	NuclAT_0	-	Antitoxin
H7R05_RS23020	57735..57887	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
H7R05_RS23025	57959..58210	-	252	WP_001291964.1	hypothetical protein	-
H7R05_RS23030	58869..59045	-	177	WP_001054900.1	hypothetical protein	-
H7R05_RS23035	59377..59586	+	210	WP_000062602.1	HEAT repeat domain-containing protein	-
H7R05_RS23040	59658..60320	-	663	WP_000644792.1	plasmid IncI1-type surface exclusion protein ExcA	-
H7R05_RS23045	60385..62553	-	2169	WP_182913037.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55	-	1..103781	103781

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T33873 WP_001331364.1 NZ_AP021945:57735-57887 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T33873 NZ_AP021945:57735-57887 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT33873 NZ_AP021945:c57680-57623 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin