Detailed information of TA system TA033825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2332670..2333308
• Replicon: chromosome
• Accession: NZ_AP021935
• Organism: Escherichia coli strain WP2-W18-ESBL-10

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: H7X62_RS10915
• Protein ID: WP_000813795.1
• Coordinates: 2332670..2332846 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H7X62_RS10920
• Protein ID: WP_076797675.1
• Coordinates: 2332892..2333308 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7X62_RS10895	2328292..2329503	-	1212	WP_071591517.1	BenE family transporter YdcO	-
H7X62_RS10900	2329556..2330092	+	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
H7X62_RS10905	2330165..2332126	+	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H7X62_RS10910	2332218..2332448	-	231	WP_023910283.1	YncJ family protein	-
H7X62_RS10915	2332670..2332846	+	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H7X62_RS10920	2332892..2333308	+	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H7X62_RS10925	2333387..2334793	+	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
H7X62_RS10930	2335038..2336183	+	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
H7X62_RS10935	2336201..2337214	+	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
H7X62_RS10940	2337215..2338156	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T33825 WP_000813795.1 NZ_AP021935:2332670-2332846 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T33825 NZ_AP021935:2332670-2332846 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT33825 WP_076797675.1 NZ_AP021935:2332892-2333308 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT33825 NZ_AP021935:2332892-2333308 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1