Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4083068..4083904 Replicon chromosome
Accession NZ_AP021893
Organism Escherichia coli strain 2018-06-4CC

Toxin (Protein)


Gene name yeeV Uniprot ID A0A5E2RWI0
Locus tag HRR20_RS19705 Protein ID WP_001559913.1
Coordinates 4083068..4083445 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NQ68
Locus tag HRR20_RS19710 Protein ID WP_001540478.1
Coordinates 4083536..4083904 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR20_RS19675 4078275..4079381 + 1107 WP_001353974.1 N-acetylneuraminate epimerase -
HRR20_RS19680 4079446..4080426 + 981 WP_001295601.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
HRR20_RS19685 4080434..4081084 - 651 WP_001037966.1 HNH endonuclease -
HRR20_RS19690 4082129..4082281 - 153 WP_021581278.1 hypothetical protein -
HRR20_RS19695 4082366..4082563 - 198 WP_021581277.1 DUF957 domain-containing protein -
HRR20_RS19700 4082583..4083071 - 489 WP_021581276.1 hypothetical protein -
HRR20_RS19705 4083068..4083445 - 378 WP_001559913.1 TA system toxin CbtA family protein Toxin
HRR20_RS19710 4083536..4083904 - 369 WP_001540478.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HRR20_RS19715 4084061..4084282 - 222 WP_000692300.1 DUF987 domain-containing protein -
HRR20_RS19720 4084351..4084827 - 477 WP_001186774.1 RadC family protein -
HRR20_RS19725 4084843..4085316 - 474 WP_021581273.1 antirestriction protein -
HRR20_RS19730 4085410..4085655 - 246 WP_152922235.1 antirestriction protein -
HRR20_RS19735 4085655..4086476 - 822 WP_001519291.1 DUF945 domain-containing protein -
HRR20_RS19740 4086844..4087326 - 483 WP_001519290.1 hypothetical protein -
HRR20_RS19745 4087687..4088229 - 543 WP_001519289.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB 4075483..4095491 20008


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14148.10 Da        Isoelectric Point: 7.8276

>T33610 WP_001559913.1 NZ_AP021893:c4083445-4083068 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T33610 NZ_AP021893:c4083445-4083068 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGACCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13652.53 Da        Isoelectric Point: 7.8398

>AT33610 WP_001540478.1 NZ_AP021893:c4083904-4083536 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT33610 NZ_AP021893:c4083904-4083536 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E2RWI0

Antitoxin

Source ID Structure
AlphaFold DB S1NQ68

References