Detailed information of TA system TA033585

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3643832..3644667
• Replicon: chromosome
• Accession: NZ_AP021892
• Organism: Escherichia coli strain 2018-01-1CC

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A763DSF8
• Locus tag: HRR21_RS17370
• Protein ID: WP_047928871.1
• Coordinates: 3644290..3644667 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HRR21_RS17365
• Protein ID: WP_047928872.1
• Coordinates: 3643832..3644200 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRR21_RS17340	3640948..3641766	+	819	WP_001234702.1	DUF945 domain-containing protein	-
HRR21_RS17345	3641857..3642342	+	486	WP_000214316.1	antirestriction protein	-
HRR21_RS17350	3642357..3642833	+	477	WP_148251817.1	RadC family protein	-
HRR21_RS17355	3642902..3643123	+	222	WP_000692353.1	DUF987 domain-containing protein	-
HRR21_RS17360	3643138..3643782	+	645	WP_101975700.1	antitoxin of toxin-antitoxin stability system	-
HRR21_RS17365	3643832..3644200	+	369	WP_047928872.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HRR21_RS17370	3644290..3644667	+	378	WP_047928871.1	TA system toxin CbtA family protein	Toxin
HRR21_RS17375	3644664..3644813	+	150	Protein_3384	hypothetical protein	-
HRR21_RS17380	3644889..3645086	+	198	WP_096093965.1	DUF957 domain-containing protein	-
HRR21_RS17385	3645171..3646016	+	846	WP_072650597.1	DUF4942 domain-containing protein	-
HRR21_RS17395	3646316..3646822	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
HRR21_RS17400	3646901..3648742	-	1842	WP_000437375.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13941.88 Da
• Isoelectric Point: 7.3222

>T33585 WP_047928871.1 NZ_AP021892:3644290-3644667 [Escherichia coli]
MKTLPVLPGQAAGSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRTVNGITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T33585 NZ_AP021892:3644290-3644667 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCGGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCATTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCACCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGGCATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13757.47 Da
• Isoelectric Point: 5.0907

>AT33585 WP_047928872.1 NZ_AP021892:3643832-3644200 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEDNQLHYLADRAGIRGRFSDADAYHLDQALPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT33585 NZ_AP021892:3643832-3644200 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGACTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAAGACAACCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAAGCCCTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A763DSF8