Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ypjF-yeeU/CbtA-CbeA
Location 1083485..1084217 Replicon chromosome
Accession NZ_AP021891
Organism Escherichia coli strain 2017.01.04CC

Toxin (Protein)


Gene name ypjF Uniprot ID A0A377CSV0
Locus tag VEGS_RS05195 Protein ID WP_001194227.1
Coordinates 1083485..1083814 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A377CUM4
Locus tag VEGS_RS05200 Protein ID WP_001059335.1
Coordinates 1083852..1084217 (-) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VEGS_RS05190 1078551..1083125 + 4575 WP_124038348.1 adhesin-like autotransporter YpjA/EhaD -
VEGS_RS05195 1083485..1083814 - 330 WP_001194227.1 TA system toxin CbtA family protein Toxin
VEGS_RS05200 1083852..1084217 - 366 WP_001059335.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
VEGS_RS05205 1084243..1084464 - 222 WP_000691984.1 DUF987 domain-containing protein -
VEGS_RS05210 1084461..1085003 - 543 WP_001098974.1 DNA repair protein RadC -
VEGS_RS05215 1085016..1085459 - 444 WP_000381004.1 antirestriction protein -
VEGS_RS05220 1085490..1086311 - 822 WP_001234410.1 DUF945 domain-containing protein -
VEGS_RS05225 1086403..1086744 - 342 WP_000720953.1 hypothetical protein -
VEGS_RS05230 1086779..1087243 - 465 WP_000791791.1 hypothetical protein -
VEGS_RS05235 1087369..1087527 - 159 WP_001128372.1 hypothetical protein -
VEGS_RS05240 1087749..1088636 - 888 WP_001567853.1 50S ribosome-binding GTPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(17-117)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12445.11 Da        Isoelectric Point: 7.2767

>T33550 WP_001194227.1 NZ_AP021891:c1083814-1083485 [Escherichia coli]
MQTLSSHPTRATQPCLSPVETWQRLLTHLLSQHYGLTLNDTPFSNETTIREDIDAGVSLSDAVNFLVEKYELVRIDRKGF
SWQEQTPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T33550 NZ_AP021891:c1083814-1083485 [Escherichia coli]
ATGCAAACCTTATCATCACACCCGACAAGGGCGACTCAGCCCTGTCTGTCACCCGTTGAAACCTGGCAACGGCTGCTGAC
TCATCTGTTATCGCAGCACTATGGCCTCACGCTGAATGACACACCGTTCAGTAATGAAACCACCATACGTGAAGATATAG
ATGCCGGAGTATCGCTCAGCGATGCGGTGAACTTTCTGGTCGAAAAATACGAACTGGTTCGAATCGACCGAAAGGGATTT
TCGTGGCAAGAACAAACCCCGTATATTTCCGTAGTGGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13545.40 Da        Isoelectric Point: 5.8806

>AT33550 WP_001059335.1 NZ_AP021891:c1084217-1083852 [Escherichia coli]
MNNHSVFGTKPENLTCQEWGLKRTITPCFGARLVQEGNRVHFLADRAGFNGAFIDDNALRLDLAFPLILKQLELMLTSGE
LSPRHEHCVTLYHNGLTCEADTLGSCGYVYIAIYPDQPEPH

Download         Length: 366 bp

>AT33550 NZ_AP021891:c1084217-1083852 [Escherichia coli]
ATGAACAATCACTCAGTATTCGGTACCAAACCGGAGAATCTTACCTGCCAGGAGTGGGGACTAAAGCGCACGATCACCCC
CTGTTTTGGCGCTCGTCTGGTGCAGGAAGGTAACCGGGTGCATTTTCTCGCTGACAGGGCCGGATTCAACGGTGCTTTCA
TCGACGATAACGCGCTACGCCTGGATCTGGCCTTTCCGTTGATACTCAAGCAACTGGAGCTGATGCTCACCAGCGGAGAA
CTCTCTCCCCGGCATGAGCACTGCGTCACGCTATATCACAATGGACTGACTTGCGAAGCCGATACACTCGGCTCCTGTGG
CTATGTCTACATCGCTATTTATCCCGACCAGCCTGAACCCCATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377CSV0

Antitoxin

Source ID Structure
AlphaFold DB A0A377CUM4

References