Detailed information of TA system TA033525

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2002332..2002703
• Replicon: chromosome
• Accession: NZ_AP021890
• Organism: Escherichia coli strain 2017.15.01CC

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: HRR22_RS09460
• Protein ID: WP_001317028.1
• Coordinates: 2002509..2002703 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2002332..2002510 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRR22_RS09430	1998084..1998257	+	174	WP_001296046.1	hypothetical protein	-
HRR22_RS09435	1998287..1999660	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
HRR22_RS09440	1999789..2000724	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
HRR22_RS09445	2000776..2002011	-	1236	WP_000040858.1	site-specific integrase	-
HRR22_RS09450	2002013..2002228	-	216	WP_000079604.1	excisionase XisR	-
HRR22_RS09455	2002307..2002516	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2002332..2002510	+	179	NuclAT_0	-	Antitoxin
-	2002332..2002510	+	179	NuclAT_0	-	Antitoxin
-	2002332..2002510	+	179	NuclAT_0	-	Antitoxin
-	2002332..2002510	+	179	NuclAT_0	-	Antitoxin
HRR22_RS09460	2002509..2002703	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
HRR22_RS09465	2002760..2003569	-	810	WP_000166319.1	recombination protein RecT	-
HRR22_RS09470	2003562..2006153	-	2592	WP_000105142.1	exodeoxyribonuclease VIII	-
HRR22_RS09475	2006255..2006530	-	276	WP_000632297.1	protein RacC	-
HRR22_RS09480	2006605..2006775	-	171	WP_001352098.1	conserved protein, Rac prophage	-
HRR22_RS09485	2006775..2006996	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2000776..2055837	55061

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T33525 WP_001317028.1 NZ_AP021890:c2002703-2002509 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T33525 NZ_AP021890:c2002703-2002509 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT33525 NZ_AP021890:2002332-2002510 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin