Detailed information of TA system TA033188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 461511..462348
• Replicon: chromosome
• Accession: NZ_AP019856
• Organism: Escherichia coli O8:H8 strain 16F5M1D1

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: EcoO8H8_RS02060
• Protein ID: WP_000227784.1
• Coordinates: 461511..462053 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: EcoO8H8_RS02065
• Protein ID: WP_001297137.1
• Coordinates: 462037..462348 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EcoO8H8_RS02040	457050..457961	-	912	WP_000705853.1	2-dehydropantoate 2-reductase	-
EcoO8H8_RS02045	458129..458620	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
EcoO8H8_RS02050	458748..460112	-	1365	WP_001000978.1	MFS transporter	-
EcoO8H8_RS02055	460520..461455	+	936	WP_096306785.1	sel1 repeat family protein	-
EcoO8H8_RS02060	461511..462053	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
EcoO8H8_RS02065	462037..462348	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
EcoO8H8_RS02070	462533..463423	-	891	WP_000971336.1	heme o synthase	-
EcoO8H8_RS02075	463435..463764	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
EcoO8H8_RS02080	463764..464378	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
EcoO8H8_RS02085	464368..466359	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
EcoO8H8_RS02090	466381..467328	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T33188 WP_000227784.1 NZ_AP019856:c462053-461511 [Escherichia coli O8:H8]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T33188 NZ_AP019856:c462053-461511 [Escherichia coli O8:H8]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATATGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT33188 WP_001297137.1 NZ_AP019856:c462348-462037 [Escherichia coli O8:H8]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT33188 NZ_AP019856:c462348-462037 [Escherichia coli O8:H8]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3