Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 9808..10333 Replicon plasmid pTMSNI47-3
Accession NZ_AP019690
Organism Klebsiella quasipneumoniae strain SNI47

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag TMSI_RS29450 Protein ID WP_001568026.1
Coordinates 10028..10333 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag TMSI_RS29445 Protein ID WP_001568025.1
Coordinates 9808..10026 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TMSI_RS29410 5018..7915 + 2898 WP_001553819.1 Tn3-like element Tn5403 family transposase -
TMSI_RS29415 7948..8220 - 273 Protein_9 IS6 family transposase -
TMSI_RS29420 8267..8503 - 237 WP_001348075.1 AAA family ATPase -
TMSI_RS29425 8577..8993 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
TMSI_RS29430 8990..9220 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
TMSI_RS29435 9177..9638 + 462 WP_172969442.1 hypothetical protein -
TMSI_RS29445 9808..10026 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
TMSI_RS29450 10028..10333 + 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
TMSI_RS29455 10502..10897 + 396 WP_072158608.1 hypothetical protein -
TMSI_RS29460 10924..11247 + 324 WP_022644730.1 hypothetical protein -
TMSI_RS29465 11244..12260 + 1017 WP_020315256.1 hypothetical protein -
TMSI_RS29470 12595..13863 - 1269 WP_019705993.1 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..89834 89834
- inside IScluster/Tn - - 3847..13863 10016


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T32903 WP_001568026.1 NZ_AP019690:10028-10333 [Klebsiella quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T32903 NZ_AP019690:10028-10333 [Klebsiella quasipneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT32903 WP_001568025.1 NZ_AP019690:9808-10026 [Klebsiella quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT32903 NZ_AP019690:9808-10026 [Klebsiella quasipneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References