Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 8577..9220 Replicon plasmid pTMSNI47-3
Accession NZ_AP019690
Organism Klebsiella quasipneumoniae strain SNI47

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag TMSI_RS29425 Protein ID WP_001044770.1
Coordinates 8577..8993 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag TMSI_RS29430 Protein ID WP_001261282.1
Coordinates 8990..9220 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TMSI_RS29400 3847..4284 - 438 Protein_6 IS6-like element IS26 family transposase -
TMSI_RS29405 4318..4923 - 606 WP_000509966.1 recombinase family protein -
TMSI_RS29410 5018..7915 + 2898 WP_001553819.1 Tn3-like element Tn5403 family transposase -
TMSI_RS29415 7948..8220 - 273 Protein_9 IS6 family transposase -
TMSI_RS29420 8267..8503 - 237 WP_001348075.1 AAA family ATPase -
TMSI_RS29425 8577..8993 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
TMSI_RS29430 8990..9220 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
TMSI_RS29435 9177..9638 + 462 WP_172969442.1 hypothetical protein -
TMSI_RS29445 9808..10026 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
TMSI_RS29450 10028..10333 + 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB -
TMSI_RS29455 10502..10897 + 396 WP_072158608.1 hypothetical protein -
TMSI_RS29460 10924..11247 + 324 WP_022644730.1 hypothetical protein -
TMSI_RS29465 11244..12260 + 1017 WP_020315256.1 hypothetical protein -
TMSI_RS29470 12595..13863 - 1269 WP_019705993.1 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..89834 89834
- inside IScluster/Tn - - 3847..13863 10016


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T32902 WP_001044770.1 NZ_AP019690:c8993-8577 [Klebsiella quasipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T32902 NZ_AP019690:c8993-8577 [Klebsiella quasipneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT32902 WP_001261282.1 NZ_AP019690:c9220-8990 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT32902 NZ_AP019690:c9220-8990 [Klebsiella quasipneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References