Detailed information of TA system TA032899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 15275..15932
• Replicon: plasmid pTMSNI47-1
• Accession: NZ_AP019688
• Organism: Klebsiella quasipneumoniae strain SNI47

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: TMSI_RS27375
• Protein ID: WP_000270043.1
• Coordinates: 15582..15932 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: TMSI_RS27370
• Protein ID: WP_000124640.1
• Coordinates: 15275..15577 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TMSI_RS27320	10493..11305	+	813	WP_151819882.1	3'-5' exonuclease	-
TMSI_RS27325	11299..11745	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
TMSI_RS27330	11742..12260	+	519	WP_011872844.1	hypothetical protein	-
TMSI_RS27335	12260..12490	+	231	WP_020833601.1	hypothetical protein	-
TMSI_RS27340	12477..13334	+	858	WP_001167033.1	hypothetical protein	-
TMSI_RS27350	13565..14092	+	528	WP_011872875.1	thermonuclease family protein	-
TMSI_RS27355	14146..14583	+	438	WP_151819883.1	ATP-binding protein	-
TMSI_RS27360	14593..14865	+	273	WP_151819884.1	HU family DNA-binding protein	-
TMSI_RS27365	14954..15247	+	294	WP_020833606.1	chromosome segregation protein ParM	-
TMSI_RS27370	15275..15577	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
TMSI_RS27375	15582..15932	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TMSI_RS27380	16095..16643	+	549	WP_151819885.1	transcriptional regulator	-
TMSI_RS27390	16984..17178	+	195	WP_151819886.1	hypothetical protein	-
TMSI_RS27395	17302..17565	+	264	WP_151819887.1	hypothetical protein	-
TMSI_RS27400	17791..18147	-	357	WP_020833609.1	hypothetical protein	-
TMSI_RS27405	18244..18732	+	489	WP_164597181.1	DUF1643 domain-containing protein	-
TMSI_RS27410	18735..19232	+	498	WP_000062185.1	hypothetical protein	-
TMSI_RS27420	20091..20573	+	483	Protein_32	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / dfrA14 / blaCTX-M-2 / qnrB4 / blaDHA-1 / blaKHM-1	-	1..185311	185311

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T32899 WP_000270043.1 NZ_AP019688:c15932-15582 [Klebsiella quasipneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T32899 NZ_AP019688:c15932-15582 [Klebsiella quasipneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT32899 WP_000124640.1 NZ_AP019688:c15577-15275 [Klebsiella quasipneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT32899 NZ_AP019688:c15577-15275 [Klebsiella quasipneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCTGACGGCACCCACTACGGTTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9