Detailed information of TA system TA032896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2758533..2760121
• Replicon: chromosome
• Accession: NZ_AP019687
• Organism: Klebsiella quasipneumoniae strain SNI47

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: TMSI_RS13880
• Protein ID: WP_032452828.1
• Coordinates: 2758533..2759855 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2N4W0X3
• Locus tag: TMSI_RS13885
• Protein ID: WP_023289667.1
• Coordinates: 2759855..2760121 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TMSI_RS13850	2753578..2755233	-	1656	WP_101861587.1	EAL domain-containing protein	-
TMSI_RS13855	2755531..2755779	+	249	WP_004205912.1	hypothetical protein	-
TMSI_RS13860	2756071..2756289	+	219	WP_002904808.1	ParD-like family protein	-
TMSI_RS13865	2756270..2757052	+	783	WP_048297502.1	type I methionyl aminopeptidase	-
TMSI_RS13875	2757224..2758516	+	1293	WP_101861586.1	glycoside hydrolase family 10 protein	-
TMSI_RS13880	2758533..2759855	-	1323	WP_032452828.1	type II toxin-antitoxin system HipA family toxin	Toxin
TMSI_RS13885	2759855..2760121	-	267	WP_023289667.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
TMSI_RS13890	2760386..2760712	-	327	WP_032452826.1	hydroxyisourate hydrolase	-
TMSI_RS13895	2760709..2761209	-	501	WP_151819770.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
TMSI_RS13900	2761187..2762566	-	1380	WP_032452824.1	purine permease	-
TMSI_RS13905	2762912..2764066	+	1155	WP_023289663.1	FAD-dependent urate hydroxylase HpxO	-
TMSI_RS13910	2764047..2764970	-	924	WP_023289662.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49202.53 Da
• Isoelectric Point: 8.9165

>T32896 WP_032452828.1 NZ_AP019687:c2759855-2758533 [Klebsiella quasipneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDDEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEARLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATRGRKTEINAIYPRHFLATAKAVNFPRDQMLAILDEFADRVP
QAIESARRTLPADFSAHVWRAITENMLKLHARLQQGLQAR

Nucleotide

• Length: 1323 bp

>T32896 NZ_AP019687:c2759855-2758533 [Klebsiella quasipneumoniae]
ATGGCGACGCTCACTACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGACGAGTGGTTGCGCAGCCCGCGCGCCAGGCCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCACAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAACAGCCGTTCGATCTGCTGGCCGAGGTCGGCCGGGATAGCGTAGGGGCGGTGACTCTGCTGCCGCCGGG
AGAAGAGGCGCGTCTTGCAGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAACTGACCGCGTTGCTGACCGCCTACCAGT
CGGATATTCCGCTCGGGATGGTGACCGGCCAGGACGATTTTCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACCGCGCTG
CTGCGAATGGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATTGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATCTGTGCCTGGCGCTGGCCAGGGCGTTGG
GCCTGGCGGTGCCGGAGGCGGAGATTATCACCACCCCGCGGATCCGTGCGCTGGCGGTGACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGGCTGCCGCAGGAGGATCTGTGTCAGGCATTCGGCATCCCGTCGTCGATGAAATA
CGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAATTTCTCGCTCTTTTTGCTG
CCCGGCGGCAGCTACCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGAGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAACGCCACCCGAGGGCGGAAAACGGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTTCCCCGGGATCAGATGCTGGCGATCCTTGACGAGTTTGCCGACCGCGTGCCG
CAGGCCATCGAAAGCGCCCGCCGAACGCTGCCGGCGGACTTTTCCGCCCACGTCTGGCGGGCGATCACGGAGAACATGCT
GAAACTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9891.25 Da
• Isoelectric Point: 7.3163

>AT32896 WP_023289667.1 NZ_AP019687:c2760121-2759855 [Klebsiella quasipneumoniae]
MHSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETATAP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT32896 NZ_AP019687:c2760121-2759855 [Klebsiella quasipneumoniae]
ATGCATTCCCCGCAGATATACAGTCCCGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCACCCGGACAAAACCACCTTGA
CCACCCTGTTCAAGATCCTCCAGTCGCTGGGGGTGGTGATGACCCTGAACGCCAAGCCGGCCGAGACGGCGACGGCCCCC
GATAACACCACGGATCTGGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2N4W0X3