Detailed information of TA system TA032881

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 120515..120941
• Replicon: plasmid pGSH8M-2-1
• Accession: NZ_AP019676
• Organism: Escherichia coli strain GSH8M-2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GDK32_RS23750
• Protein ID: WP_001312861.1
• Coordinates: 120515..120673 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	120717..120941 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDK32_RS23705	115561..115788	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
GDK32_RS23710	115882..116568	-	687	WP_000332484.1	PAS domain-containing protein	-
GDK32_RS23715	116759..117142	-	384	WP_000124981.1	relaxosome protein TraM	-
GDK32_RS23720	117419..118066	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
GDK32_RS23725	118363..119184	-	822	WP_001234469.1	DUF945 domain-containing protein	-
GDK32_RS23730	119306..119593	-	288	WP_000107535.1	hypothetical protein	-
GDK32_RS23735	119618..119824	-	207	WP_000547939.1	hypothetical protein	-
GDK32_RS23750	120515..120673	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	120717..120941	-	225	NuclAT_0	-	Antitoxin
-	120717..120941	-	225	NuclAT_0	-	Antitoxin
-	120717..120941	-	225	NuclAT_0	-	Antitoxin
-	120717..120941	-	225	NuclAT_0	-	Antitoxin
GDK32_RS25280	120753..120941	+	189	WP_001299721.1	hypothetical protein	-
GDK32_RS23755	120953..121672	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
GDK32_RS23760	121669..122103	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
GDK32_RS23765	122158..124116	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
GDK32_RS23770	124175..124408	-	234	WP_000006004.1	DUF905 domain-containing protein	-
GDK32_RS23775	124464..124991	-	528	WP_000290793.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / blaTEM-1C / dfrA14 / qnrS1 / floR / sitABCD	iroB / iroC / iroD / iroE / iroN	1..145389	145389

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T32881 WP_001312861.1 NZ_AP019676:c120673-120515 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T32881 NZ_AP019676:c120673-120515 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT32881 NZ_AP019676:c120941-120717 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin