Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 13075..13676 Replicon plasmid pE2863-2
Accession NZ_AP018804
Organism Escherichia coli strain E2863

Toxin (Protein)


Gene name doc Uniprot ID I2USK8
Locus tag EL381_RS28165 Protein ID WP_001216042.1
Coordinates 13075..13455 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID I2USI4
Locus tag EL381_RS28170 Protein ID WP_001190710.1
Coordinates 13455..13676 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL381_RS28130 8106..9134 + 1029 WP_001292232.1 tyrosine-type recombinase/integrase -
EL381_RS28135 9208..9552 + 345 WP_164717617.1 hypothetical protein -
EL381_RS28140 9549..10025 + 477 WP_000861171.1 hypothetical protein -
EL381_RS28145 10022..10516 + 495 WP_000640905.1 dUTP diphosphatase -
EL381_RS28150 10531..11181 + 651 WP_001108662.1 DUF2829 domain-containing protein -
EL381_RS28155 11188..11955 + 768 WP_000446290.1 hypothetical protein -
EL381_RS28160 11945..13039 + 1095 WP_000201931.1 hypothetical protein -
EL381_RS28165 13075..13455 - 381 WP_001216042.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
EL381_RS28170 13455..13676 - 222 WP_001190710.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EL381_RS28175 13749..14138 - 390 WP_000506730.1 DNA repair protein -
EL381_RS28180 14226..14510 - 285 WP_001142392.1 hypothetical protein -
EL381_RS28185 14494..15243 - 750 WP_000196958.1 hypothetical protein -
EL381_RS28190 15240..15449 - 210 WP_000190275.1 hypothetical protein -
EL381_RS28195 15474..15767 - 294 WP_000267999.1 hypothetical protein -
EL381_RS28200 15791..16072 - 282 WP_000002100.1 ASCH domain-containing protein -
EL381_RS30235 16065..16289 - 225 Protein_21 DUF551 domain-containing protein -
EL381_RS30240 16482..16691 - 210 Protein_22 hypothetical protein -
EL381_RS28210 16688..16897 - 210 WP_000951712.1 hypothetical protein -
EL381_RS28215 16899..17087 - 189 WP_085948948.1 hypothetical protein -
EL381_RS30040 17257..17433 - 177 WP_001261533.1 hypothetical protein -
EL381_RS28220 17433..17912 - 480 WP_000182372.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..92925 92925


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.6406

>T32351 WP_001216042.1 NZ_AP018804:c13455-13075 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELYGSNI

Download         Length: 381 bp

>T32351 NZ_AP018804:c13455-13075 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCAACCTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCTGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAACTATATGGTTCCAATATTTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8147.13 Da        Isoelectric Point: 4.7708

>AT32351 WP_001190710.1 NZ_AP018804:c13676-13455 [Escherichia coli]
MQSINFRTARGNLSELLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT32351 NZ_AP018804:c13676-13455 [Escherichia coli]
ATGCAGTCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAATTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAACCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAGGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2USK8

Antitoxin

Source ID Structure
AlphaFold DB A0A348ERB2

References