Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1553394..1554038 Replicon chromosome
Accession NZ_AP018780
Organism Haemophilus influenzae strain CHBN-IV-1 isolate 13H36

Toxin (Protein)


Gene name hicA Uniprot ID Q4QKY0
Locus tag K6J73_RS07660 Protein ID WP_005643265.1
Coordinates 1553394..1553579 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q4QKX9
Locus tag K6J73_RS07665 Protein ID WP_005692602.1
Coordinates 1553610..1554038 (+) Length 143 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J73_RS07625 (CHBNIV1_14480) 1549102..1549992 - 891 WP_050847652.1 phage antirepressor N-terminal domain-containing protein -
K6J73_RS07630 (CHBNIV1_14500) 1550546..1550782 - 237 WP_050847651.1 hypothetical protein -
K6J73_RS07635 (CHBNIV1_14510) 1550803..1550994 - 192 WP_050847650.1 hypothetical protein -
K6J73_RS07640 (CHBNIV1_14520) 1551153..1551800 + 648 WP_050847649.1 hypothetical protein -
K6J73_RS07645 (CHBNIV1_14530) 1551874..1552599 - 726 WP_221252938.1 hypothetical protein -
K6J73_RS07650 (CHBNIV1_14540) 1552592..1552957 - 366 WP_005672809.1 RusA family crossover junction endodeoxyribonuclease -
K6J73_RS07655 (CHBNIV1_14550) 1552954..1553238 - 285 WP_046950508.1 DUF1364 domain-containing protein -
K6J73_RS07660 1553394..1553579 + 186 WP_005643265.1 type II toxin-antitoxin system HicA family toxin Toxin
K6J73_RS07665 (CHBNIV1_14560) 1553610..1554038 + 429 WP_005692602.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
K6J73_RS07670 (CHBNIV1_14570) 1554039..1554548 - 510 WP_005692600.1 DUF1367 family protein -
K6J73_RS07675 (CHBNIV1_14580) 1554553..1555143 - 591 WP_042600466.1 DNA N-6-adenine-methyltransferase -
K6J73_RS07680 (CHBNIV1_14590) 1555140..1555775 - 636 WP_112083855.1 replication protein P -
K6J73_RS07685 (CHBNIV1_14600) 1555760..1556518 - 759 WP_221252939.1 helix-turn-helix domain-containing protein -
K6J73_RS07690 (CHBNIV1_14610) 1556515..1557189 - 675 WP_038441201.1 phage antirepressor KilAC domain-containing protein -
K6J73_RS07695 (CHBNIV1_14620) 1557238..1557678 - 441 WP_005651303.1 YmfL family putative regulatory protein -
K6J73_RS07700 (CHBNIV1_14630) 1557727..1557930 - 204 WP_005651305.1 YdaS family helix-turn-helix protein -
K6J73_RS07705 (CHBNIV1_14640) 1558052..1558807 + 756 WP_005668731.1 S24 family peptidase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1511456..1574734 63278


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 62 a.a.        Molecular weight: 6716.88 Da        Isoelectric Point: 11.1595

>T32209 WP_005643265.1 NZ_AP018780:1553394-1553579 [Haemophilus influenzae]
MRSSDLIKELKSAGCTFVRHGKGDHQIWQSPITGKTFPVPHPKQHVPIGTLRSIKKSAGLL

Download         Length: 186 bp

>T32209 NZ_AP018780:1553394-1553579 [Haemophilus influenzae]
ATGCGATCCAGCGACTTAATCAAGGAACTTAAAAGTGCAGGTTGTACTTTTGTCAGGCACGGTAAAGGCGATCATCAAAT
CTGGCAATCGCCCATCACAGGGAAGACGTTCCCCGTACCGCATCCAAAACAACACGTACCCATCGGCACATTAAGATCCA
TCAAAAAATCGGCAGGGCTTCTATAG

Antitoxin


Download         Length: 143 a.a.        Molecular weight: 16458.45 Da        Isoelectric Point: 4.3241

>AT32209 WP_005692602.1 NZ_AP018780:1553610-1554038 [Haemophilus influenzae]
MLFTIGIETPTNENEAYGITVPALFTEEYSCFSAADTLEEIPTQVTDAIHSILEMMFEDGIDINELQDKGYRHYQTQEDF
NYCDTWLLLDVDISAYQGKRHRINISLPEYLIKRIDSRVASNPIYKDRSHFLAIASQKELRQ

Download         Length: 429 bp

>AT32209 NZ_AP018780:1553610-1554038 [Haemophilus influenzae]
ATGTTATTCACTATCGGCATTGAAACCCCAACAAACGAAAATGAAGCCTACGGCATTACGGTGCCAGCATTATTTACTGA
AGAATATTCCTGCTTTAGTGCCGCTGATACCCTTGAGGAAATCCCAACGCAGGTGACTGATGCCATTCATTCCATCTTAG
AAATGATGTTTGAAGACGGAATAGACATCAACGAACTTCAAGATAAAGGCTATCGTCACTACCAAACGCAAGAAGATTTC
AACTATTGCGATACTTGGTTGTTGCTTGATGTAGATATTTCCGCATATCAAGGCAAACGCCACCGTATTAATATCAGCTT
GCCTGAATACCTTATCAAACGTATTGATAGCCGTGTTGCAAGCAATCCAATCTACAAAGACCGCAGCCACTTTTTAGCGA
TTGCCTCACAAAAAGAGCTACGGCAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB Q4QKY0


Antitoxin

Source ID Structure
AlphaFold DB Q4QKX9

References