Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 46318..46949 Replicon chromosome
Accession NZ_AP018776
Organism Haemophilus influenzae strain CHBN-III-5 isolate 13H30

Toxin (Protein)


Gene name vapC Uniprot ID Q4QLW0
Locus tag K6J67_RS00225 Protein ID WP_005651560.1
Coordinates 46551..46949 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q4QLV9
Locus tag K6J67_RS00220 Protein ID WP_005648011.1
Coordinates 46318..46551 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J67_RS00195 (CHBNIII5_00400) 41797..42999 - 1203 WP_050822922.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
K6J67_RS00200 (CHBNIII5_00410) 43162..43827 + 666 WP_013527272.1 DNA repair protein RadC -
K6J67_RS00205 (CHBNIII5_00420) 44041..44277 + 237 WP_005542826.1 50S ribosomal protein L28 -
K6J67_RS00210 (CHBNIII5_00430) 44289..44459 + 171 WP_005613503.1 50S ribosomal protein L33 -
K6J67_RS00215 (CHBNIII5_00440) 44807..46171 + 1365 WP_014550999.1 diaminobutyrate--2-oxoglutarate transaminase -
K6J67_RS00220 (CHBNIII5_00450) 46318..46551 + 234 WP_005648011.1 type II toxin-antitoxin system antitoxin VapB2 Antitoxin
K6J67_RS00225 (CHBNIII5_00460) 46551..46949 + 399 WP_005651560.1 tRNA(fMet)-specific endonuclease VapC Toxin
K6J67_RS00230 (CHBNIII5_00470) 46969..48504 + 1536 WP_011272306.1 L-2,4-diaminobutyrate decarboxylase -
K6J67_RS00235 (CHBNIII5_00480) 48736..49551 + 816 WP_011272305.1 DNA-formamidopyrimidine glycosylase -
K6J67_RS00240 (CHBNIII5_00490) 49625..50647 - 1023 WP_005651554.1 outer membrane-stress sensor serine endopeptidase DegS -
K6J67_RS00245 (CHBNIII5_00500) 50648..51766 - 1119 WP_005686201.1 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

SpoVT-AbrB domain

(7-56)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15115.46 Da        Isoelectric Point: 8.0514

>T32176 WP_005651560.1 NZ_AP018776:46551-46949 [Haemophilus influenzae]
VLKYMLDTNIVIYVIKRRPLEILSRFNQNAGKMCVSSITVAELYYGAEKSEYPERNIAVIEDFLSRLTILDYQPKHAAHF
GNIKAELSKQGKLIGENDIHIAAHARSEGLILVSNNLREFERVIALRTENWV

Download         Length: 399 bp

>T32176 NZ_AP018776:46551-46949 [Haemophilus influenzae]
GTGTTGAAATATATGCTTGATACTAACATTGTGATTTACGTGATTAAGCGTAGGCCATTGGAAATTCTTTCTCGCTTTAA
TCAAAATGCAGGAAAAATGTGCGTGAGTAGCATTACTGTGGCTGAACTCTATTATGGAGCTGAAAAAAGCGAATATCCCG
AAAGAAACATTGCGGTTATTGAAGATTTCTTATCACGCCTCACCATTTTAGATTACCAACCAAAGCATGCAGCTCATTTC
GGCAATATTAAAGCTGAACTCTCTAAACAAGGGAAATTGATTGGCGAAAATGATATTCATATAGCTGCTCATGCTCGTAG
TGAAGGGTTAATTCTGGTTAGTAACAATTTAAGAGAATTTGAGCGAGTTATAGCATTACGTACAGAAAATTGGGTGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8880.98 Da        Isoelectric Point: 4.4013

>AT32176 WP_005648011.1 NZ_AP018776:46318-46551 [Haemophilus influenzae]
MIEASVFMTNRSQAVRLPAEVRFSEEIKKLSVRVSGSDRILSPLNQSWDSFFLNDQAVSDDFMNEREIAFQPEREAL

Download         Length: 234 bp

>AT32176 NZ_AP018776:46318-46551 [Haemophilus influenzae]
ATGATTGAAGCCTCTGTTTTTATGACTAATCGAAGTCAAGCTGTGCGTTTGCCCGCTGAAGTTCGCTTTTCTGAAGAAAT
CAAAAAATTATCGGTTCGTGTATCGGGCAGTGATCGGATTTTATCGCCTCTCAACCAATCTTGGGATAGTTTTTTCTTGA
ATGATCAAGCAGTTAGCGATGATTTTATGAATGAACGAGAAATTGCATTTCAACCAGAGCGTGAGGCTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q4QLW0

Antitoxin

Source ID Structure
AlphaFold DB A0A806DGS0

References