Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3097376..3097946 Replicon chromosome
Accession NZ_AP018034
Organism Mycobacterium tuberculosis strain HN-205

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag CPZ09_RS15235 Protein ID WP_003414166.1
Coordinates 3097376..3097732 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag CPZ09_RS15240 Protein ID WP_003901465.1
Coordinates 3097716..3097946 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ09_RS15215 3092828..3094516 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
CPZ09_RS15220 3094520..3094846 - 327 WP_003414157.1 hypothetical protein -
CPZ09_RS15225 3095019..3095606 + 588 WP_003914429.1 DUF3558 family protein -
CPZ09_RS15230 3095625..3097274 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
CPZ09_RS15235 3097376..3097732 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
CPZ09_RS15240 3097716..3097946 - 231 WP_003901465.1 antitoxin MazE Antitoxin
CPZ09_RS15245 3097989..3099032 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
CPZ09_RS15250 3099223..3099498 + 276 WP_003911993.1 DUF1778 domain-containing protein -
CPZ09_RS21810 3099674..3099928 - 255 WP_003917684.1 hypothetical protein -
CPZ09_RS15260 3100076..3100480 + 405 WP_003414181.1 hypothetical protein -
CPZ09_RS15265 3100477..3100668 + 192 WP_003414184.1 hypothetical protein -
CPZ09_RS15270 3100732..3102021 + 1290 Protein_2887 transposase family protein -
CPZ09_RS15275 3102255..3102512 + 258 WP_003899489.1 hypothetical protein -
CPZ09_RS15280 3102617..3102928 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T31559 WP_003414166.1 NZ_AP018034:c3097732-3097376 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T31559 NZ_AP018034:c3097732-3097376 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT31559 WP_003901465.1 NZ_AP018034:c3097946-3097716 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT31559 NZ_AP018034:c3097946-3097716 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References