Detailed information of TA system TA031545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2244158..2244784
• Replicon: chromosome
• Accession: NZ_AP018034
• Organism: Mycobacterium tuberculosis strain HN-205

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: CPZ09_RS10850
• Protein ID: WP_003410075.1
• Coordinates: 2244386..2244784 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: CPZ09_RS10845
• Protein ID: WP_003911750.1
• Coordinates: 2244158..2244385 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ09_RS10835	2242197..2242541	-	345	WP_096474167.1	ferredoxin family protein	-
CPZ09_RS10840	2242730..2243899	-	1170	WP_003899126.1	ATP-binding protein	-
CPZ09_RS10845	2244158..2244385	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CPZ09_RS10850	2244386..2244784	+	399	WP_003410075.1	PIN domain nuclease	Toxin
CPZ09_RS10855	2244967..2245398	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
CPZ09_RS10860	2245499..2245933	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
CPZ09_RS10875	2246370..2246549	-	180	Protein_2061	hypothetical protein	-
CPZ09_RS10880	2246610..2247257	+	648	WP_072450434.1	transposase	-
CPZ09_RS10885	2247211..2247801	+	591	WP_003899131.1	IS110 family transposase	-
CPZ09_RS10890	2247929..2249185	-	1257	WP_003902247.1	HNH endonuclease	-
CPZ09_RS10900	2249539..2249739	+	201	Protein_2065	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T31545 WP_003410075.1 NZ_AP018034:2244386-2244784 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T31545 NZ_AP018034:2244386-2244784 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT31545 WP_003911750.1 NZ_AP018034:2244158-2244385 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT31545 NZ_AP018034:2244158-2244385 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8