Detailed information of TA system TA031531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1379559..1380247
• Replicon: chromosome
• Accession: NZ_AP018034
• Organism: Mycobacterium tuberculosis strain HN-205

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: CPZ09_RS06750
• Protein ID: WP_003406304.1
• Coordinates: 1379816..1380247 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CPZ09_RS06745
• Protein ID: WP_096474146.1
• Coordinates: 1379559..1379819 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ09_RS06725	1375136..1375960	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
CPZ09_RS06730	1375965..1377146	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
CPZ09_RS06735	1377223..1378323	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
CPZ09_RS06740	1378494..1379483	+	990	WP_003406301.1	malate dehydrogenase	-
CPZ09_RS06745	1379559..1379819	+	261	WP_096474146.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
CPZ09_RS06750	1379816..1380247	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
CPZ09_RS06755	1380270..1381853	-	1584	WP_009940130.1	PE family protein	-
CPZ09_RS06765	1382033..1382893	+	861	WP_003900301.1	hypothetical protein	-
CPZ09_RS06770	1382974..1383804	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
CPZ09_RS06775	1383861..1384154	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
CPZ09_RS06780	1384151..1384420	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T31531 WP_003406304.1 NZ_AP018034:1379816-1380247 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T31531 NZ_AP018034:1379816-1380247 [Mycobacterium tuberculosis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9932.28 Da
• Isoelectric Point: 6.4755

>AT31531 WP_096474146.1 NZ_AP018034:1379559-1379819 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAAFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT31531 NZ_AP018034:1379559-1379819 [Mycobacterium tuberculosis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGCCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0THR7