Detailed information of TA system TA031527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1008562..1009181
• Replicon: chromosome
• Accession: NZ_AP018034
• Organism: Mycobacterium tuberculosis strain HN-205

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: CPZ09_RS04905
• Protein ID: WP_003404726.1
• Coordinates: 1008747..1009181 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: CPZ09_RS04900
• Protein ID: WP_003898641.1
• Coordinates: 1008562..1008741 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ09_RS04890	1004035..1005615	+	1581	WP_003910977.1	serine hydrolase	-
CPZ09_RS04895	1005612..1008005	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
CPZ09_RS21710	1008278..1008436	+	159	WP_003404720.1	hypothetical protein	-
CPZ09_RS04900	1008562..1008741	+	180	WP_003898641.1	antitoxin	Antitoxin
CPZ09_RS04905	1008747..1009181	+	435	WP_003404726.1	SRPBCC family protein	Toxin
CPZ09_RS04910	1009279..1010052	+	774	WP_003404735.1	VOC family protein	-
CPZ09_RS04915	1010117..1010566	+	450	WP_003404738.1	hypothetical protein	-
CPZ09_RS04920	1010701..1010931	-	231	WP_003898642.1	hypothetical protein	-
CPZ09_RS04925	1011098..1012606	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
CPZ09_RS04930	1012608..1013846	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T31527 WP_003404726.1 NZ_AP018034:1008747-1009181 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T31527 NZ_AP018034:1008747-1009181 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT31527 WP_003898641.1 NZ_AP018034:1008562-1008741 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT31527 NZ_AP018034:1008562-1008741 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5