Detailed information of TA system TA030981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 929835..930327
• Replicon: chromosome
• Accession: NZ_AP014862
• Organism: Pseudomonas furukawaii strain KF707

Toxin (Protein)

• Gene name: -
• Uniprot ID: L8MF62
• Locus tag: KF707C_RS04330
• Protein ID: WP_004420135.1
• Coordinates: 929835..930107 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: L8M941
• Locus tag: KF707C_RS04335
• Protein ID: WP_004420134.1
• Coordinates: 930088..930327 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF707C_RS04310	925051..925989	+	939	WP_004420140.1	bifunctional riboflavin kinase/FAD synthetase	-
KF707C_RS04315	926005..928854	+	2850	WP_004420139.1	isoleucine--tRNA ligase	-
KF707C_RS04320	928847..929350	+	504	WP_004420138.1	signal peptidase II	-
KF707C_RS04325	929343..929783	+	441	WP_004420136.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
KF707C_RS04330	929835..930107	+	273	WP_004420135.1	BrnT family toxin	Toxin
KF707C_RS04335	930088..930327	+	240	WP_004420134.1	hypothetical protein	Antitoxin
KF707C_RS04340	930431..931375	+	945	WP_004420133.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
KF707C_RS04345	931432..931851	-	420	WP_192817691.1	type IV pilin protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10620.22 Da
• Isoelectric Point: 8.0261

>T30981 WP_004420135.1 NZ_AP014862:929835-930107 [Pseudomonas furukawaii]
MLRFEYDEAKSLANRVKHGIDFMTAQQLWNDPDLLEIPARTLDEPRYLVIARLLDRCWSAVITYRHGRIRIISVRRSRVE
EVSLYESSGI

Nucleotide

• Length: 273 bp

>T30981 NZ_AP014862:929835-930107 [Pseudomonas furukawaii]
ATGCTGCGATTTGAGTACGACGAGGCCAAGAGCCTTGCCAACCGGGTCAAGCACGGCATCGACTTCATGACTGCGCAGCA
GCTGTGGAACGATCCGGACCTGTTGGAAATTCCGGCCAGGACGCTGGATGAGCCGCGATACCTGGTGATCGCCCGCCTGC
TGGATAGGTGCTGGTCGGCGGTGATCACTTACCGCCACGGACGAATTCGCATTATTTCCGTCCGCCGTTCACGGGTAGAG
GAGGTCAGTCTTTATGAAAGCAGTGGAATTTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9317.53 Da
• Isoelectric Point: 5.1617

>AT30981 WP_004420134.1 NZ_AP014862:930088-930327 [Pseudomonas furukawaii]
MKAVEFDRLFDEEEGDVLEHLDLAQAKRSGHEQKRVNVDFPVWMIESLDREASRLGVTRQSIIKIWLAERLEQVTHRQS

Nucleotide

• Length: 240 bp

>AT30981 NZ_AP014862:930088-930327 [Pseudomonas furukawaii]
ATGAAAGCAGTGGAATTTGACCGCCTGTTCGATGAAGAGGAAGGCGATGTTCTCGAACATCTCGACCTGGCGCAGGCGAA
GCGTTCCGGGCATGAGCAGAAGCGGGTGAATGTGGATTTTCCCGTCTGGATGATCGAGTCGCTGGATCGGGAGGCCAGCC
GCCTGGGCGTCACGCGTCAGTCGATCATCAAGATCTGGCTGGCGGAACGCCTGGAGCAAGTCACACACCGGCAGTCCTGA

Structures

Toxin

• AlphaFold DB: L8MF62

Antitoxin

• AlphaFold DB: L8M941