Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3222978..3223705 Replicon chromosome
Accession NZ_AP014857
Organism Escherichia albertii strain EC06-170

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag AST80_RS15665 Protein ID WP_000550189.1
Coordinates 3223391..3223705 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AST80_RS15660 Protein ID WP_000560272.1
Coordinates 3222978..3223394 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AST80_RS15650 3218285..3220636 + 2352 WP_060877472.1 alpha-glucosidase -
AST80_RS15655 3220870..3222888 + 2019 WP_060877473.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
AST80_RS15660 3222978..3223394 - 417 WP_000560272.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
AST80_RS15665 3223391..3223705 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
AST80_RS15670 3223988..3225124 - 1137 WP_060877474.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
AST80_RS15675 3225209..3225712 + 504 WP_059225049.1 M48 family metallopeptidase -
AST80_RS15680 3225789..3226481 + 693 WP_000942533.1 vancomycin high temperature exclusion protein -
AST80_RS15685 3226560..3227546 + 987 WP_000617693.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T30971 WP_000550189.1 NZ_AP014857:c3223705-3223391 [Escherichia albertii]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T30971 NZ_AP014857:c3223705-3223391 [Escherichia albertii]
ATGCACCTGATCACTCAAAAAGCATTGAAAGATGCAGCGGAAAAATATCCGCAGCATAAAACGGAGTTGGTGGCACTGGG
GAACACGATTGCTAAGGGATACTTCAAAAAACCTGAGTCATTAAAAGCAGTGTTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCACAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14993.40 Da        Isoelectric Point: 4.3697

>AT30971 WP_000560272.1 NZ_AP014857:c3223394-3222978 [Escherichia albertii]
MIAIADILQAGEKLTDVAPFLAGIQSEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMVQAMPGG
VAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLNGKRKLTLEHAKKLAARFGISPALFID

Download         Length: 417 bp

>AT30971 NZ_AP014857:c3223394-3222978 [Escherichia albertii]
ATGATTGCGATTGCCGATATCTTGCAAGCAGGAGAAAAGCTAACCGATGTGGCACCTTTTCTGGCGGGTATCCAGAGCGA
AGAACAGTATGCCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAATCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCGATGGTTCAAGCCATGCCTGGCGGT
GTTGCTGTGATTCGTACCCTGATGGATCAATACGGCTTAACCCTTTCCGATCTTCCAGAAATTGGCAGTAAATCCATGGT
GTCACGCGTTTTGAACGGGAAGAGAAAATTAACGCTGGAACACGCTAAAAAACTGGCAGCGCGATTCGGCATTTCTCCTG
CGTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References