Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3175038..3175675 Replicon chromosome
Accession NZ_AP014704
Organism Methylobacterium aquaticum strain MA-22A

Toxin (Protein)


Gene name vapC Uniprot ID A0A1I2IG95
Locus tag Maq22A_RS14515 Protein ID WP_060847569.1
Coordinates 3175038..3175451 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name VapB1 Uniprot ID A0A1I2IL36
Locus tag Maq22A_RS14520 Protein ID WP_060849416.1
Coordinates 3175448..3175675 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Maq22A_RS14500 3171535..3172410 - 876 WP_060847566.1 branched-chain amino acid ABC transporter permease -
Maq22A_RS14505 3172531..3173691 - 1161 WP_060847567.1 ABC transporter substrate-binding protein -
Maq22A_RS14510 3173946..3175016 + 1071 WP_060847568.1 malate/lactate/ureidoglycolate dehydrogenase -
Maq22A_RS14515 3175038..3175451 - 414 WP_060847569.1 type II toxin-antitoxin system VapC family toxin Toxin
Maq22A_RS14520 3175448..3175675 - 228 WP_060849416.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
Maq22A_RS14525 3175823..3176842 + 1020 WP_082742549.1 SMP-30/gluconolactonase/LRE family protein -
Maq22A_RS14530 3177163..3177912 - 750 WP_060847570.1 SDR family oxidoreductase -
Maq22A_RS14535 3177932..3178588 - 657 WP_060847571.1 ATP-binding cassette domain-containing protein -
Maq22A_RS14540 3178575..3179345 - 771 WP_060847572.1 ABC transporter ATP-binding protein -
Maq22A_RS14545 3179342..3180274 - 933 WP_060847573.1 branched-chain amino acid ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-126)

Antitoxin

SpoVT-AbrB domain

(7-43)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14933.23 Da        Isoelectric Point: 9.7212

>T30878 WP_060847569.1 NZ_AP014704:c3175451-3175038 [Methylobacterium aquaticum]
VKPRYLLDTNVIIALRRRRPRSVVARLTALRIGEAVMSPVTYGELCFGAEKSADRDNAFAVLARLVAVVPIEIAEPQETG
RRYGEIRATLGRQGQLIGNNDVWIAAHALAANLTLVTGNVGEFSRVPGLVIEDWAAQ

Download         Length: 414 bp

>T30878 NZ_AP014704:c3175451-3175038 [Methylobacterium aquaticum]
GTGAAGCCTCGCTACCTCCTCGATACGAACGTCATCATCGCGCTCAGGCGCCGGCGGCCACGCTCGGTCGTTGCTCGGTT
GACGGCACTCCGCATCGGCGAAGCCGTCATGTCGCCAGTCACTTACGGCGAGCTGTGCTTTGGGGCGGAGAAGAGCGCGG
ACCGGGACAACGCTTTCGCAGTCCTCGCCCGGCTGGTCGCGGTCGTTCCGATCGAGATCGCCGAGCCCCAGGAAACCGGC
CGCCGCTATGGTGAAATCCGGGCCACGCTCGGACGACAAGGGCAGCTGATCGGCAACAACGATGTCTGGATCGCCGCGCA
CGCGCTCGCGGCGAACCTTACGCTCGTGACCGGCAATGTCGGTGAGTTCAGCCGAGTGCCCGGTCTCGTCATCGAGGATT
GGGCGGCCCAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8459.68 Da        Isoelectric Point: 4.1964

>AT30878 WP_060849416.1 NZ_AP014704:c3175675-3175448 [Methylobacterium aquaticum]
MTVAKLFMSGGSQAVRLPREFRFDGSEVEIFRRGDEVVLRERPANLSVLLDIIDDMPADMLIDLQDGPPQEREGL

Download         Length: 228 bp

>AT30878 NZ_AP014704:c3175675-3175448 [Methylobacterium aquaticum]
ATGACTGTTGCCAAGCTCTTCATGTCCGGAGGCAGCCAGGCCGTCAGACTGCCGCGAGAGTTCCGATTCGACGGCAGCGA
GGTCGAGATTTTTCGCCGTGGCGACGAAGTCGTTCTGCGCGAGCGCCCGGCCAATCTCTCCGTGCTCCTCGACATCATCG
ACGACATGCCCGCCGACATGCTGATCGATCTGCAGGATGGCCCGCCGCAGGAGCGCGAGGGCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I2IG95

Antitoxin

Source ID Structure
AlphaFold DB A0A1I2IL36

References