Detailed information of TA system TA030770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 15752..16268
• Replicon: chromosome
• Accession: NZ_AP014623
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain StFRB508

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A8A9Z863
• Locus tag: PCAU_RS00060
• Protein ID: WP_028681510.1
• Coordinates: 15987..16268 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: PCAU_RS00055
• Protein ID: WP_023969916.1
• Coordinates: 15752..15997 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCAU_RS00030	11520..12515	+	996	WP_096372733.1	sulfotransferase family protein	-
PCAU_RS00035	12481..13239	-	759	WP_038583164.1	slipin family protein	-
PCAU_RS00040	13241..14626	-	1386	WP_096372734.1	nodulation protein NfeD	-
PCAU_RS00045	14850..15311	+	462	WP_016705004.1	YbaK/EbsC family protein	-
PCAU_RS00050	15442..15687	+	246	WP_023969915.1	DUF2789 domain-containing protein	-
PCAU_RS00055	15752..15997	+	246	WP_023969916.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
PCAU_RS00060	15987..16268	+	282	WP_028681510.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PCAU_RS00065	16401..16829	+	429	WP_096372735.1	winged helix DNA-binding protein	-
PCAU_RS00070	16826..18910	+	2085	WP_096372736.1	FUSC family protein	-
PCAU_RS00075	18907..19116	+	210	WP_025808541.1	DUF1656 domain-containing protein	-
PCAU_RS00080	19113..20009	+	897	WP_096372737.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (8-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10907.82 Da
• Isoelectric Point: 10.6258

>T30770 WP_028681510.1 NZ_AP014623:15987-16268 [Pseudomonas chlororaphis subsp. aurantiaca]
MTYKLQFLPSARKEWDKLGHTLREQFKRKLAERLEMPRVPADALHGMADCYKIKLKASGYRLVYQVIEDQVVVSVVAVGK
RERSAVYERARKR

Nucleotide

• Length: 282 bp

>T30770 NZ_AP014623:15987-16268 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGACTTATAAGCTCCAGTTTCTGCCTTCGGCGCGCAAGGAATGGGACAAGCTCGGTCATACCCTGCGCGAGCAATTCAA
GAGGAAGCTGGCCGAGCGCCTGGAAATGCCACGGGTGCCCGCGGACGCCCTTCACGGTATGGCGGACTGCTACAAGATCA
AGCTCAAGGCCTCTGGCTATCGCCTGGTTTACCAAGTGATCGAGGATCAGGTCGTGGTTTCGGTGGTGGCCGTGGGCAAA
CGTGAACGCAGTGCCGTGTATGAGCGGGCACGAAAGCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8840.18 Da
• Isoelectric Point: 4.7164

>AT30770 WP_023969916.1 NZ_AP014623:15752-15997 [Pseudomonas chlororaphis subsp. aurantiaca]
MQSVLADKAVSVSELKKNPSAVLNGAQGGPVAVLNHNRVMGYMVPADVFEAMMERLDDLELAEIVRARRHETPVPVNLDD
L

Nucleotide

• Length: 246 bp

>AT30770 NZ_AP014623:15752-15997 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCAAAGCGTATTGGCTGATAAAGCTGTCAGCGTGTCTGAACTGAAAAAAAATCCTTCGGCTGTCCTGAACGGCGCACA
GGGTGGTCCGGTTGCGGTGCTCAATCACAACCGGGTCATGGGCTATATGGTCCCGGCCGACGTGTTTGAAGCCATGATGG
AGCGTCTTGACGATCTTGAACTGGCGGAGATTGTCCGTGCCCGTCGTCACGAGACCCCGGTCCCGGTAAATCTGGATGAC
TTATAA

Structures

Toxin

• AlphaFold DB: A0A8A9Z863