Detailed information of TA system TA030621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1700482..1701048
• Replicon: chromosome
• Accession: NZ_AP014520
• Organism: Aggregatibacter actinomycetemcomitans NUM4039 strain NUM 4039

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JU35
• Locus tag: AANUM_RS08515
• Protein ID: WP_005543444.1
• Coordinates: 1700770..1701048 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: AANUM_RS08510
• Protein ID: WP_005543446.1
• Coordinates: 1700482..1700754 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AANUM_RS08485 (AANUM_1713)	1697019..1697285	-	267	WP_005543457.1	oxaloacetate decarboxylase subunit gamma	-
AANUM_RS12340 (AANUM_1714)	1697649..1697840	+	192	WP_005553960.1	hypothetical protein	-
AANUM_RS08490 (AANUM_1715)	1697815..1698192	+	378	WP_005543455.1	hypothetical protein	-
AANUM_RS08495 (AANUM_1716)	1698313..1698588	-	276	WP_005561117.1	hydrogenase maturation factor HybG	-
AANUM_RS08500 (AANUM_1717)	1698618..1698905	-	288	WP_005566275.1	[NiFe]-hydrogenase assembly chaperone HybE	-
AANUM_RS08505 (AANUM_1718)	1699044..1700473	-	1430	Protein_1703	bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF	-
AANUM_RS08510 (AANUM_1720)	1700482..1700754	-	273	WP_005543446.1	HigA family addiction module antitoxin	Antitoxin
AANUM_RS08515 (AANUM_1721)	1700770..1701048	-	279	WP_005543444.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AANUM_RS08520 (AANUM_1722)	1701143..1702144	-	1002	WP_005543441.1	anthranilate phosphoribosyltransferase	-
AANUM_RS08525 (AANUM_1723)	1702156..1702548	-	393	WP_005543439.1	tautomerase family protein	-
AANUM_RS08530 (AANUM_1724)	1702622..1703212	-	591	WP_005543437.1	aminodeoxychorismate/anthranilate synthase component II	-
AANUM_RS08535 (AANUM_1725)	1703223..1704770	-	1548	WP_005543435.1	anthranilate synthase component 1	-
AANUM_RS08540 (AANUM_1726)	1704936..1705490	-	555	WP_005543432.1	HAD family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-79)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 9.5983

>T30621 WP_005543444.1 NZ_AP014520:c1701048-1700770 [Aggregatibacter actinomycetemcomitans NUM4039]
MILSFKHKGLEQFFKTGSTAGIQAKHAKKLNLQLATLNNAETPLAMSVPSWNLHKLKGDLQEHWAVTVNGNWRLTFKFEN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T30621 NZ_AP014520:c1701048-1700770 [Aggregatibacter actinomycetemcomitans NUM4039]
ATGATTTTATCTTTCAAACATAAGGGACTTGAACAATTTTTTAAAACCGGTTCGACCGCAGGAATTCAAGCCAAACACGC
CAAGAAATTAAATCTGCAATTGGCCACATTAAATAATGCGGAAACGCCTCTTGCTATGAGTGTTCCCAGCTGGAATTTGC
ACAAACTTAAAGGTGATTTGCAAGAGCATTGGGCAGTTACCGTAAACGGAAATTGGCGCTTAACATTTAAATTTGAAAAT
GGTCATGCTGAAGTTGTTGATTATCAGGATTATCATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10138.74 Da
• Isoelectric Point: 7.3169

>AT30621 WP_005543446.1 NZ_AP014520:c1700754-1700482 [Aggregatibacter actinomycetemcomitans NUM4039]
MHNPPHPGELLKEYIDGVSVTKVAQKLGVSRVTLSRILNGKASITPEMAVRLSELLNTTTPKLWLGMQADYDLWQIEQQH
AVFNIQPLFN

Nucleotide

• Length: 273 bp

>AT30621 NZ_AP014520:c1700754-1700482 [Aggregatibacter actinomycetemcomitans NUM4039]
ATGCACAATCCTCCACACCCGGGAGAACTGTTAAAAGAATATATTGATGGCGTGAGTGTCACGAAGGTTGCCCAAAAATT
AGGTGTTTCGCGTGTTACGCTTTCCCGCATTCTTAATGGGAAAGCAAGTATAACGCCTGAAATGGCTGTGCGATTAAGCG
AATTATTGAATACCACAACACCGAAATTATGGCTGGGTATGCAAGCAGACTACGATTTATGGCAAATTGAACAACAACAC
GCCGTATTCAACATCCAACCATTATTTAATTAA

Structures

Toxin

• AlphaFold DB: X2JU35