Detailed information of TA system TA030618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 573308..573896
• Replicon: chromosome
• Accession: NZ_AP014520
• Organism: Aggregatibacter actinomycetemcomitans NUM4039 strain NUM 4039

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5D0ELR5
• Locus tag: AANUM_RS02950
• Protein ID: WP_005541261.1
• Coordinates: 573621..573896 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AANUM_RS02945
• Protein ID: WP_005541263.1
• Coordinates: 573308..573604 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AANUM_RS02920 (AANUM_0596)	568707..569750	-	1044	WP_005541273.1	glycosyltransferase family 9 protein	-
AANUM_RS02925 (AANUM_0597)	569839..570564	+	726	WP_060828734.1	3-deoxy-D-manno-octulosonic acid kinase	-
AANUM_RS02930 (AANUM_0598)	570613..571107	-	495	WP_005541270.1	pantetheine-phosphate adenylyltransferase	-
AANUM_RS02935 (AANUM_0599)	571108..572364	-	1257	WP_005595140.1	lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase	-
AANUM_RS02940 (AANUM_0600)	572489..573250	+	762	WP_005541265.1	glycosyltransferase family 2 protein	-
AANUM_RS02945 (AANUM_0601)	573308..573604	-	297	WP_005541263.1	HigA family addiction module antitoxin	Antitoxin
AANUM_RS02950 (AANUM_0602)	573621..573896	-	276	WP_005541261.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AANUM_RS02955 (AANUM_0603)	574013..575380	-	1368	WP_005541259.1	CDP-diacylglycerol--serine O-phosphatidyltransferase	-
AANUM_RS02960 (AANUM_0604)	575566..576642	+	1077	WP_005551449.1	tRNA/rRNA methyltransferase	-
AANUM_RS02965 (AANUM_0605)	576709..576969	-	261	WP_032998892.1	YfhL family 4Fe-4S dicluster ferredoxin	-
AANUM_RS02970 (AANUM_0606)	577037..577732	+	696	WP_005541254.1	ribonuclease T2 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-91)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-85)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10595.09 Da
• Isoelectric Point: 8.0554

>T30618 WP_005541261.1 NZ_AP014520:c573896-573621 [Aggregatibacter actinomycetemcomitans NUM4039]
MIKSFKHKGLQAYFETGVTKGIQSAHKKKINGILDLIDNATSETEFKMFYQCHPLKGDREGIWSMKVSGNWRITFEFING
DAYILNYEDYH

Nucleotide

• Length: 276 bp

>T30618 NZ_AP014520:c573896-573621 [Aggregatibacter actinomycetemcomitans NUM4039]
ATGATTAAGAGTTTTAAACATAAAGGATTACAAGCCTATTTTGAAACCGGTGTAACCAAAGGGATTCAATCTGCACACAA
AAAGAAAATCAACGGTATTCTTGATTTAATTGATAATGCAACTTCTGAAACGGAATTTAAGATGTTTTACCAATGCCATC
CGTTAAAAGGTGACAGAGAAGGAATTTGGTCAATGAAAGTATCGGGTAATTGGCGTATTACTTTTGAGTTTATTAACGGT
GATGCGTATATATTAAACTATGAAGATTATCATTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11099.77 Da
• Isoelectric Point: 7.4038

>AT30618 WP_005541263.1 NZ_AP014520:c573604-573308 [Aggregatibacter actinomycetemcomitans NUM4039]
MRKPAHPGEILLDGFIEPHDIKIKDLAEHLGFSRETLSRVIHGKTPMTANLALALEDAGISTARLWLSLQSNYDVWALKQ
TREASTIRPFVFSEQRFV

Nucleotide

• Length: 297 bp

>AT30618 NZ_AP014520:c573604-573308 [Aggregatibacter actinomycetemcomitans NUM4039]
ATGCGTAAACCTGCACATCCGGGCGAAATTTTATTAGACGGTTTTATTGAACCACATGACATTAAGATTAAAGATTTAGC
CGAGCATTTAGGTTTTTCCCGTGAAACGCTTTCGCGGGTCATTCACGGCAAAACGCCAATGACTGCAAATTTAGCGTTGG
CATTGGAGGATGCCGGCATCAGTACCGCCCGTTTATGGCTTTCATTACAAAGCAATTATGATGTTTGGGCATTGAAACAA
ACACGGGAAGCATCCACAATCCGGCCTTTTGTATTCTCGGAACAACGTTTTGTATAA

Structures

Toxin

• AlphaFold DB: A0A5D0ELR5