Detailed information of TA system TA030547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-HTH_XRE
• Location: 1262877..1264177
• Replicon: chromosome
• Accession: NZ_AP012332
• Organism: Gardnerella vaginalis ATCC 14018 = JCM 11026

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: GAVG_RS04945
• Protein ID: WP_004113001.1
• Coordinates: 1262877..1263686 (-)
• Length: 270 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: S4HGB9
• Locus tag: GAVG_RS04950
• Protein ID: WP_004113003.1
• Coordinates: 1263704..1264177 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAVG_RS04925	1258779..1259516	+	738	WP_004112993.1	thiamine diphosphokinase	-
GAVG_RS04930	1259692..1260747	+	1056	WP_004112995.1	type I glyceraldehyde-3-phosphate dehydrogenase	-
GAVG_RS04935	1260858..1261358	+	501	WP_004112997.1	Cys-tRNA(Pro) deacylase	-
GAVG_RS04940	1261406..1262365	+	960	WP_004112999.1	aldose 1-epimerase family protein	-
GAVG_RS04945	1262877..1263686	-	810	WP_004113001.1	zeta toxin family protein	Toxin
GAVG_RS04950	1263704..1264177	-	474	WP_004113003.1	helix-turn-helix domain-containing protein	Antitoxin
GAVG_RS04955	1264715..1266151	-	1437	WP_009994216.1	hypothetical protein	-
GAVG_RS04960	1267011..1268021	+	1011	WP_004116755.1	UDP-glucose 4-epimerase GalE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (34-221)

Antitoxin

• Cro/C1-type helix-turn-helix domain (6-59)

Sequences

Toxin

Protein

• Length: 270 a.a.
• Molecular weight: 30905.69 Da
• Isoelectric Point: 8.9574

>T30547 WP_004113001.1 NZ_AP012332:c1263686-1262877 [Gardnerella vaginalis ATCC 14018 = JCM 11026]
MGKCDFAMVIEDFSEEYFTHCLLRNLRSLQMYKHADLHPKAVLLGGQSGAGKTTIHVIKQKEFNGNIIILDADSYRTQHP
YYCELLDKYGKESVKYTSAFSAKMVRSLIDELSSKKYNLIIEGTLRSVEVPSSTAKMLIDRGYRISLAVIATKPELSWIS
TLIRYREMYLEDPKSARATPKDHHDNIVNNIVDNLLKLEQSKIFDRIQIYKRDEKCIYDSDSYKNSPNITAASVLKEVLF
GKKTIDEKKLICHAKNRLNELDKLIDKSL

Nucleotide

• Length: 810 bp

>T30547 NZ_AP012332:c1263686-1262877 [Gardnerella vaginalis ATCC 14018 = JCM 11026]
ATGGGTAAGTGTGATTTTGCTATGGTTATAGAAGATTTTAGCGAAGAGTATTTTACTCACTGTTTGTTAAGAAATTTACG
TTCTTTACAAATGTATAAGCATGCAGATTTGCATCCTAAGGCGGTTTTACTCGGTGGTCAAAGTGGTGCTGGCAAAACGA
CCATTCATGTAATAAAACAAAAAGAATTTAACGGTAACATAATAATCCTTGATGCTGATAGCTATAGGACTCAACACCCT
TATTATTGCGAGTTGCTTGATAAATACGGTAAAGAAAGTGTTAAATATACGAGTGCTTTTTCAGCAAAAATGGTGAGAAG
CTTAATTGATGAGTTAAGTTCTAAAAAATATAATCTCATAATTGAAGGTACTTTGCGCAGTGTTGAAGTTCCTAGTAGTA
CTGCGAAAATGTTGATAGATCGTGGTTACCGTATTTCTCTTGCTGTCATTGCAACAAAACCTGAATTGTCATGGATCAGT
ACGCTGATTCGCTACAGGGAAATGTATTTGGAAGATCCTAAAAGTGCTAGGGCAACTCCTAAGGATCATCACGACAATAT
TGTGAACAATATAGTAGATAACTTGTTGAAATTAGAACAATCGAAAATATTTGACAGAATTCAGATTTACAAGCGAGACG
AAAAATGTATATATGATTCTGATTCGTATAAAAATAGTCCAAATATTACAGCAGCTAGTGTTCTTAAGGAAGTGCTCTTT
GGCAAGAAAACTATTGATGAGAAAAAGTTGATTTGCCACGCTAAGAATAGGTTGAATGAACTAGACAAGTTGATAGATAA
AAGTTTGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 18016.48 Da
• Isoelectric Point: 5.3394

>AT30547 WP_004113003.1 NZ_AP012332:c1264177-1263704 [Gardnerella vaginalis ATCC 14018 = JCM 11026]
MVSVNIRALRRSKKITQQELAEALGVSRNSIVRYENGTSAISTRLIDKICDKFKVSLFDIVHEKELSASDKYDLNMKIEV
LKERGALLLARLYKYEDSMNIQFDDSSNAWIAISDDLSDVINTKIYTSTNFEDINRYIGYLDGIERLLEYVSCNKNS

Nucleotide

• Length: 474 bp

>AT30547 NZ_AP012332:c1264177-1263704 [Gardnerella vaginalis ATCC 14018 = JCM 11026]
ATGGTTTCTGTGAATATTCGAGCACTACGTCGCTCAAAAAAAATAACTCAACAAGAGTTAGCTGAAGCATTAGGAGTATC
TCGTAATAGCATTGTGCGATACGAAAATGGTACAAGTGCTATTTCAACTAGGTTAATAGATAAGATTTGTGATAAATTCA
AGGTTTCTCTTTTTGATATCGTACATGAAAAAGAATTATCTGCATCTGATAAATACGATTTGAATATGAAGATTGAAGTT
TTAAAGGAGCGTGGCGCACTTTTACTGGCTCGTTTATATAAATACGAGGATAGTATGAATATACAATTTGATGATTCTTC
TAATGCATGGATTGCAATTAGTGATGATTTATCAGATGTAATTAATACAAAGATTTATACATCTACAAATTTTGAAGATA
TAAATCGTTATATCGGATATTTAGATGGCATAGAACGCTTGCTTGAATACGTAAGTTGCAATAAAAATAGTTAA

Structures

Antitoxin

• AlphaFold DB: S4HGB9