Detailed information of TA system TA030420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1408777..1409434
• Replicon: chromosome
• Accession: NC_023066
• Organism: Pseudomonas aeruginosa LES431

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8G3IXE4
• Locus tag: T223_RS06615
• Protein ID: WP_003098540.1
• Coordinates: 1409252..1409434 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: T223_RS06610
• Protein ID: WP_004351284.1
• Coordinates: 1408777..1409205 (-)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
T223_RS06575	1404790..1405494	+	705	WP_012613696.1	phage regulatory protein/antirepressor Ant	-
T223_RS06580	1405559..1406392	+	834	WP_012613697.1	helix-turn-helix domain-containing protein	-
T223_RS06585	1406379..1407176	+	798	WP_012613698.1	hypothetical protein	-
T223_RS06590	1407173..1407379	+	207	WP_024082445.1	hypothetical protein	-
T223_RS06595	1407376..1407957	+	582	WP_012613699.1	recombination protein NinG	-
T223_RS06600	1407954..1408250	+	297	WP_024082446.1	hypothetical protein	-
T223_RS06605	1408252..1408626	+	375	WP_004351286.1	hypothetical protein	-
T223_RS06610	1408777..1409205	-	429	WP_004351284.1	hypothetical protein	Antitoxin
T223_RS06615	1409252..1409434	-	183	WP_003098540.1	type II toxin-antitoxin system HicA family toxin	Toxin
T223_RS06620	1409635..1409949	+	315	WP_015649339.1	phage holin, lambda family	-
T223_RS06625	1409949..1410566	+	618	WP_012613700.1	glycoside hydrolase family 19 protein	-
T223_RS06630	1410566..1411036	+	471	WP_025208377.1	lysis system i-spanin subunit Rz	-
T223_RS06635	1411033..1411776	+	744	WP_012613702.1	hypothetical protein	-
T223_RS06640	1411896..1412441	+	546	WP_019486501.1	terminase small subunit	-
T223_RS06645	1412413..1414377	+	1965	WP_012613704.1	phage terminase large subunit family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1390655..1434240	43585

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (7-78)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6746.86 Da
• Isoelectric Point: 11.0775

>T30420 WP_003098540.1 NC_023066:c1409434-1409252 [Pseudomonas aeruginosa LES431]
MKFSEFRRWLKAQGVTFEAGKGSHFKITAPNGKQTTFADHGAKEMPEPTRKAIIKQLGLK

Nucleotide

• Length: 183 bp

>T30420 NC_023066:c1409434-1409252 [Pseudomonas aeruginosa LES431]
ATGAAGTTCAGCGAATTCAGACGATGGTTGAAGGCCCAAGGGGTGACCTTCGAAGCCGGCAAAGGGAGCCACTTCAAGAT
CACCGCCCCGAACGGCAAACAGACCACCTTCGCGGACCACGGAGCTAAGGAAATGCCAGAACCGACCCGCAAGGCGATCA
TCAAGCAACTGGGGCTCAAATGA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 15756.94 Da
• Isoelectric Point: 4.6562

>AT30420 WP_004351284.1 NC_023066:c1409205-1408777 [Pseudomonas aeruginosa LES431]
MYDYAIRFEQDDSAPGVAVFCRDLPELNSYGDDKVHAIGEAVDAIESTLSLYVDQRREIPAASQAQPGERVIHLPAVTVA
KIALWNEMVRRDMRKADLCRLLGIAQTQGDRLVDFLHNTKMEAMENALSALGLRLSVNIEAA

Nucleotide

• Length: 429 bp

>AT30420 NC_023066:c1409205-1408777 [Pseudomonas aeruginosa LES431]
ATGTACGACTATGCAATCCGTTTCGAACAGGACGATAGCGCTCCTGGCGTTGCCGTTTTCTGCAGAGACTTGCCGGAGCT
GAACAGCTATGGCGACGACAAGGTCCACGCAATCGGCGAGGCAGTCGACGCCATCGAGTCGACCCTCTCGCTATACGTTG
ATCAGCGCCGAGAAATCCCCGCGGCCAGCCAGGCGCAACCAGGCGAGCGCGTTATCCATCTGCCGGCAGTTACCGTTGCG
AAGATCGCGCTCTGGAACGAAATGGTCCGTCGAGATATGCGAAAGGCTGACCTCTGCCGGCTTCTCGGGATCGCACAGAC
CCAGGGCGACAGGCTCGTCGACTTCCTCCACAACACTAAGATGGAGGCCATGGAGAACGCTCTATCCGCCCTCGGACTCC
GCCTCTCAGTGAATATCGAGGCAGCATGA