Detailed information of TA system TA030392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2198249..2198814
• Replicon: chromosome
• Accession: NC_023032
• Organism: Cronobacter sakazakii CMCC 45402

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V5U0Z2
• Locus tag: P262_RS10405
• Protein ID: WP_023898792.1
• Coordinates: 2198536..2198814 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: P262_RS10400
• Protein ID: WP_032968184.1
• Coordinates: 2198249..2198536 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P262_RS10385	2193841..2194707	+	867	WP_007774909.1	ABC transporter permease	-
P262_RS10390	2194736..2195794	-	1059	WP_007774908.1	YncE family protein	-
P262_RS10395	2196072..2198189	+	2118	WP_023898790.1	TonB-dependent receptor	-
P262_RS10400	2198249..2198536	-	288	WP_032968184.1	HigA family addiction module antidote protein	Antitoxin
P262_RS10405	2198536..2198814	-	279	WP_023898792.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
P262_RS10410	2199113..2200717	+	1605	WP_007774898.1	alpha-amylase family protein	-
P262_RS10415	2200719..2201705	-	987	WP_007774884.1	SDR family oxidoreductase	-
P262_RS10420	2201817..2203751	-	1935	WP_007774882.1	HAMP domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10580.05 Da
• Isoelectric Point: 10.2970

>T30392 WP_023898792.1 NC_023032:c2198814-2198536 [Cronobacter malonaticus]
MIRSFRHKGLQRYFETGSTAGIHARHARKISLRLAVLNQAIRPADVDLPGFFLHPLTGDRKGIWAVTVSGNWRITFEFRD
GDVFIVNDEDYH

Nucleotide

• Length: 279 bp

>T30392 NC_023032:c2198814-2198536 [Cronobacter malonaticus]
ATGATCAGAAGCTTCAGGCATAAAGGCCTGCAACGCTATTTCGAAACGGGTTCCACCGCAGGCATTCATGCCCGCCATGC
CAGAAAGATTTCTCTGCGGCTGGCGGTGCTGAATCAGGCGATAAGACCCGCTGACGTCGACTTACCGGGCTTTTTCCTGC
ATCCCCTGACGGGGGATCGTAAAGGTATATGGGCTGTGACCGTCAGCGGAAACTGGCGCATCACGTTTGAGTTTCGGGAT
GGCGATGTCTTCATCGTCAACGATGAGGATTACCACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10437.04 Da
• Isoelectric Point: 8.7963

>AT30392 WP_032968184.1 NC_023032:c2198536-2198249 [Cronobacter malonaticus]
MATMFNPPHPGRLVQESMEALGLSARALAKALDVAPSTVQRLLVGKSDVSPEMALRLSAVLGSSPHVWLGMQNEYDLFHA
RQSVDFSHLQRLQRA

Nucleotide

• Length: 288 bp

>AT30392 NC_023032:c2198536-2198249 [Cronobacter malonaticus]
ATGGCAACGATGTTTAACCCACCACACCCAGGCAGGCTGGTGCAGGAATCAATGGAAGCGCTCGGCCTCAGCGCCCGCGC
GCTGGCCAAAGCGCTGGACGTCGCGCCGTCAACAGTGCAGCGGCTGCTGGTCGGCAAATCTGACGTCTCCCCGGAAATGG
CGCTGCGTCTCTCGGCAGTCCTTGGCAGCTCGCCGCATGTCTGGCTGGGTATGCAGAATGAATACGATCTCTTTCATGCG
CGCCAGTCTGTTGATTTCTCGCATCTGCAACGGTTGCAGAGGGCATAA

Structures

Toxin

• AlphaFold DB: V5U0Z2