Detailed information of TA system TA030260

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4947840..4948675
• Replicon: chromosome
• Accession: NC_022648
• Organism: Escherichia coli JJ1886

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: P423_RS24875
• Protein ID: WP_000854759.1
• Coordinates: 4948298..4948675 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: P423_RS24870
• Protein ID: WP_001295723.1
• Coordinates: 4947840..4948208 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P423_RS24835	4944955..4945113	+	159	WP_001323397.1	DUF905 family protein	-
P423_RS24845	4945268..4946086	+	819	WP_001234738.1	DUF945 domain-containing protein	-
P423_RS24850	4946428..4946901	+	474	WP_001350782.1	antirestriction protein	-
P423_RS24855	4946917..4947393	+	477	WP_001186775.1	RadC family protein	-
P423_RS24860	4947456..4947677	+	222	WP_000692345.1	DUF987 domain-containing protein	-
P423_RS24870	4947840..4948208	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
P423_RS24875	4948298..4948675	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
P423_RS24880	4948672..4949160	+	489	WP_000761690.1	hypothetical protein	-
P423_RS24885	4949177..4949353	+	177	WP_000839286.1	DUF957 domain-containing protein	-
P423_RS28785	4949459..4949656	+	198	WP_144079987.1	hypothetical protein	-
P423_RS24890	4949671..4950096	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
P423_RS24895	4950093..4950443	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
P423_RS24900	4950474..4952087	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
P423_RS24905	4952358..4953371	-	1014	WP_000107487.1	aldose 1-epimerase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T30260 WP_000854759.1 NC_022648:4948298-4948675 [Escherichia coli JJ1886]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T30260 NC_022648:4948298-4948675 [Escherichia coli JJ1886]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT30260 WP_001295723.1 NC_022648:4947840-4948208 [Escherichia coli JJ1886]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT30260 NC_022648:4947840-4948208 [Escherichia coli JJ1886]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52