Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 640699..641426 Replicon chromosome
Accession NZ_OX637964
Organism Escherichia coli O25b:H4-ST131 isolate 46

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag QQG90_RS03170 Protein ID WP_000550189.1
Coordinates 640699..641013 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QQG90_RS03175 Protein ID WP_000560269.1
Coordinates 641010..641426 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQG90_RS03150 (636856) 636856..637842 - 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -
QQG90_RS03155 (637921) 637921..638613 - 693 WP_000942551.1 vancomycin high temperature exclusion protein -
QQG90_RS03160 (638690) 638690..639193 - 504 WP_001295542.1 M48 family metallopeptidase -
QQG90_RS03165 (639278) 639278..640414 + 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
QQG90_RS03170 (640699) 640699..641013 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
QQG90_RS03175 (641010) 641010..641426 + 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
QQG90_RS03180 (641471) 641471..643489 - 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
QQG90_RS03185 (643715) 643715..646066 - 2352 WP_000695432.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T297142 WP_000550189.1 NZ_OX637964:640699-641013 [Escherichia coli O25b:H4-ST131]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT297142 WP_000560269.1 NZ_OX637964:641010-641426 [Escherichia coli O25b:H4-ST131]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References