Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3959828..3960425 Replicon chromosome
Accession NZ_OW970374
Organism Klebsiella pneumoniae isolate 101

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LQV21_RS19400 Protein ID WP_004142563.1
Coordinates 3960108..3960425 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LQV21_RS19395 Protein ID WP_004142561.1
Coordinates 3959828..3960115 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQV21_RS19365 (3955909) 3955909..3956157 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LQV21_RS19370 (3956175) 3956175..3956516 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LQV21_RS19375 (3956547) 3956547..3957662 - 1116 Protein_3812 MBL fold metallo-hydrolase -
LQV21_RS19380 (3957841) 3957841..3958422 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
LQV21_RS19385 (3958422) 3958422..3958790 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LQV21_RS19390 (3958910) 3958910..3959563 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
LQV21_RS19395 (3959828) 3959828..3960115 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LQV21_RS19400 (3960108) 3960108..3960425 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LQV21_RS19405 (3960610) 3960610..3961653 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
LQV21_RS19410 (3962320) 3962320..3963186 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
LQV21_RS19415 (3963295) 3963295..3964722 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T296280 WP_004142563.1 NZ_OW970374:c3960425-3960108 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T296280 NZ_OW970374:c3960425-3960108 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT296280 WP_004142561.1 NZ_OW970374:c3960115-3959828 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT296280 NZ_OW970374:c3960115-3959828 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References