Detailed information of TA system TA029581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1106150..1106790
• Replicon: chromosome
• Accession: NC_021577
• Organism: Pseudomonas aeruginosa RP73

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: M062_RS05240
• Protein ID: WP_003105740.1
• Coordinates: 1106380..1106790 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6X2S2
• Locus tag: M062_RS05235
• Protein ID: WP_003158175.1
• Coordinates: 1106150..1106380 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M062_RS05220	1101348..1103237	-	1890	WP_003105732.1	hypothetical protein	-
M062_RS29535	1103458..1103667	-	210	WP_003105733.1	cold-shock protein	-
M062_RS05230	1103975..1105894	-	1920	WP_004352838.1	type I DNA topoisomerase	-
M062_RS05235	1106150..1106380	+	231	WP_003158175.1	antitoxin	Antitoxin
M062_RS05240	1106380..1106790	+	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
M062_RS05245	1106812..1107072	+	261	WP_003105742.1	hypothetical protein	-
M062_RS05255	1107272..1107490	+	219	WP_003105747.1	hypothetical protein	-
M062_RS05260	1107556..1107801	-	246	WP_023102333.1	CrpP family protein	-
M062_RS05265	1107909..1108397	-	489	WP_003105748.1	single-stranded DNA-binding protein	-
M062_RS05270	1108427..1109266	-	840	WP_003105750.1	Rha family transcriptional regulator	-
M062_RS05275	1109313..1109861	-	549	WP_003105753.1	DUF3158 family protein	-
M062_RS05280	1109867..1110595	-	729	WP_003105754.1	TIGR03761 family integrating conjugative element protein	-
M062_RS05285	1110751..1110948	-	198	WP_003105756.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1023533..1126247	102714

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T29581 WP_003105740.1 NC_021577:1106380-1106790 [Pseudomonas aeruginosa RP73]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T29581 NC_021577:1106380-1106790 [Pseudomonas aeruginosa RP73]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATTGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGACTGGGTCGTCC
CAATCGTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.41 Da
• Isoelectric Point: 4.2821

>AT29581 WP_003158175.1 NC_021577:1106150-1106380 [Pseudomonas aeruginosa RP73]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVVVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT29581 NC_021577:1106150-1106380 [Pseudomonas aeruginosa RP73]
ATGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGTTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q6X2S2