Detailed information of TA system TA029504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 4641..5321
• Replicon: plasmid 2
• Accession: NC_021279
• Organism: Mycobacterium abscessus subsp. bolletii 50594

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4UIW4
• Locus tag: MASS_RS25980
• Protein ID: WP_016341421.1
• Coordinates: 4917..5321 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: R4V2I4
• Locus tag: MASS_RS25975
• Protein ID: WP_016341420.1
• Coordinates: 4641..4910 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MASS_RS25945	24..953	-	930	WP_079276906.1	C40 family peptidase	-
MASS_RS25950	1215..1724	-	510	WP_131807332.1	hypothetical protein	-
MASS_RS25955	1751..2590	-	840	WP_016341417.1	hypothetical protein	-
MASS_RS25960	2593..2856	-	264	WP_016341418.1	hypothetical protein	-
MASS_RS25965	2923..3336	-	414	WP_131807333.1	hypothetical protein	-
MASS_RS25970	3409..4551	-	1143	WP_016341419.1	DUF4226 domain-containing protein	-
MASS_RS25975	4641..4910	+	270	WP_016341420.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
MASS_RS25980	4917..5321	+	405	WP_016341421.1	type II toxin-antitoxin system VapC family toxin	Toxin
MASS_RS25985	5744..5923	+	180	WP_131807334.1	hypothetical protein	-
MASS_RS25990	6174..6599	+	426	WP_131807335.1	hypothetical protein	-
MASS_RS25995	6596..7792	-	1197	WP_016341422.1	DUF1173 family protein	-
MASS_RS26000	7812..8501	-	690	WP_041928275.1	hypothetical protein	-
MASS_RS26005	8665..9207	+	543	WP_041928277.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97240	97240

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14471.63 Da
• Isoelectric Point: 6.0787

>T29504 WP_016341421.1 NC_021279:4917-5321 [Mycobacteroides abscessus subsp. bolletii 50594]
VIYLDTSALVKLIRIEAESEALSEWLDERTHTPWITSVLAEVELPRAIRAAAPQGLAHVPLVLARFNRFEIDPVIRAAAA
AYPHRLRSLDAIHLATAQTAGAAVPLTAFVVYDQRLRQSAEDLGIITASPGAAA

Nucleotide

• Length: 405 bp

>T29504 NC_021279:4917-5321 [Mycobacteroides abscessus subsp. bolletii 50594]
GTGATCTATCTGGACACCTCGGCGCTGGTCAAGCTCATCCGGATCGAGGCAGAATCCGAAGCGCTCTCCGAATGGTTGGA
CGAGCGCACGCACACTCCGTGGATAACCTCGGTACTGGCCGAAGTCGAGCTACCCCGGGCAATCCGCGCCGCCGCCCCGC
AAGGCCTGGCCCATGTACCCCTCGTGTTGGCGCGGTTCAACCGGTTCGAAATCGATCCCGTCATCCGTGCGGCAGCGGCC
GCCTACCCTCACCGGCTGCGATCCCTCGACGCCATACACCTGGCGACAGCCCAAACAGCCGGCGCTGCGGTGCCTTTGAC
CGCTTTCGTCGTCTACGACCAGCGCTTACGCCAATCTGCCGAAGACCTCGGCATCATCACTGCTAGCCCAGGTGCAGCGG
CCTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9491.99 Da
• Isoelectric Point: 6.7252

>AT29504 WP_016341420.1 NC_021279:4641-4910 [Mycobacteroides abscessus subsp. bolletii 50594]
MGEVPIRELNQQTRKVINRVKAGEEIAVTDHGELIAHIIPAATGPLAALISAGKVLPAAQGPAPRAMIHAPDQPEAGVLL
EKLRSEERY

Nucleotide

• Length: 270 bp

>AT29504 NC_021279:4641-4910 [Mycobacteroides abscessus subsp. bolletii 50594]
ATGGGTGAAGTGCCAATACGTGAACTCAACCAACAGACCCGGAAAGTCATCAACCGTGTCAAGGCTGGCGAGGAAATCGC
CGTCACCGATCACGGCGAGCTCATCGCTCACATCATCCCGGCGGCCACCGGCCCTCTGGCCGCGTTGATCAGCGCGGGCA
AGGTCCTACCCGCGGCCCAGGGCCCGGCCCCGCGCGCCATGATCCATGCCCCCGATCAGCCCGAGGCTGGGGTATTGCTG
GAGAAGCTGCGCAGCGAGGAAAGGTATTAG

Structures

Toxin

• AlphaFold DB: R4UIW4

Antitoxin

• AlphaFold DB: R4V2I4