Detailed information of TA system TA029371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2435524..2436120
• Replicon: chromosome
• Accession: NC_021082
• Organism: Mannheimia haemolytica M42548

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: MHH_RS26365
• Protein ID: WP_006249776.1
• Coordinates: 2435524..2435748 (+)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A547EKP0
• Locus tag: MHH_RS26370
• Protein ID: WP_006249777.1
• Coordinates: 2435791..2436120 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MHH_RS26345	2431405..2432307	-	903	WP_006249771.1	DMT family transporter	-
MHH_RS26350	2432304..2432660	-	357	WP_006249772.1	dihydroneopterin aldolase	-
MHH_RS26355	2432730..2433335	+	606	WP_006249773.1	glycerol-3-phosphate 1-O-acyltransferase PlsY	-
MHH_RS26360	2433409..2435316	-	1908	WP_006249774.1	DNA topoisomerase IV subunit B	-
MHH_RS26365	2435524..2435748	+	225	WP_006249776.1	type II toxin-antitoxin system HicA family toxin	Toxin
MHH_RS26370	2435791..2436120	+	330	WP_006249777.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MHH_RS26375	2436159..2436545	-	387	WP_006249778.1	Cu(I)-responsive transcriptional regulator	-
MHH_RS26380	2436634..2436834	+	201	WP_006249779.1	heavy-metal-associated domain-containing protein	-
MHH_RS26385	2436887..2439079	+	2193	WP_006249780.1	copper-translocating P-type ATPase	-
MHH_RS26390	2439132..2439917	-	786	WP_006249781.1	tRNA pseudouridine(38-40) synthase TruA	-
MHH_RS26395	2439982..2440671	-	690	WP_006253589.1	GTP cyclohydrolase I FolE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (28-73)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 10.5597

>T29371 WP_006249776.1 NC_021082:2435524-2435748 [Mannheimia haemolytica M42548]
MVVKILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEEREGSRIAVVLFQQVRVFHRPHPNPDTDKGR

Nucleotide

• Length: 225 bp

>T29371 NC_021082:2435524-2435748 [Mannheimia haemolytica M42548]
ATGGTAGTAAAAATCCTAAAGCGGAAATCTCAAAAGACACTACAACAAATCTACACCCAGCCTATTTCTGCCAATATTAA
ATGGACAAATATAGAAGCTCTTTTTCTAGAGTTAGGAGCAGAAATAGAAGAACGAGAAGGGAGCAGAATTGCAGTTGTTT
TATTTCAACAGGTCAGAGTATTTCATCGCCCACACCCGAATCCTGATACAGATAAGGGGCGGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12312.06 Da
• Isoelectric Point: 4.7022

>AT29371 WP_006249777.1 NC_021082:2435791-2436120 [Mannheimia haemolytica M42548]
MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQLKKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSP
ELHKAAVIAATAENLSLNEWINQTLEKSV

Nucleotide

• Length: 330 bp

>AT29371 NC_021082:2435791-2436120 [Mannheimia haemolytica M42548]
ATGCGTAATATTATGGATATTAATGGTTATAAAGCTGTCATTGCTTACGATCCCGAAACAGAATTATTTCGAGGTGAATT
TATCGGATTAAATGGTGGAGCCGATTTTTATGCCGATAATGTCATTCAATTAAAAAAAGAAGGTGAACTAAGTTTGAATA
TCTTTTTAGAACTTTGTAGAGAAAAAGGTATTGAGCCGTTCAAACAATATTCCGGAAAGTTTAATGTTCGCCTTTCGCCT
GAATTACATAAAGCTGCGGTAATTGCAGCAACTGCTGAAAATTTAAGCTTAAATGAATGGATTAACCAAACGTTAGAAAA
GAGCGTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A547EKP0