Detailed information of TA system TA292721

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 17994..18420
• Replicon: plasmid 2
• Accession: NZ_LS992167
• Organism: Escherichia coli isolate Escherichia coli str. TO6

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECTO6_RS23875
• Protein ID: WP_001312861.1
• Coordinates: 17994..18152 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	18196..18420 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECTO6_RS23835	13364..14053	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
ECTO6_RS23840	14240..14623	-	384	WP_001151524.1	relaxosome protein TraM	-
ECTO6_RS23845	14944..15546	+	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
ECTO6_RS23850	15843..16664	-	822	WP_001234469.1	DUF945 domain-containing protein	-
ECTO6_RS23855	16785..17072	-	288	WP_000107535.1	hypothetical protein	-
ECTO6_RS25120	17097..17303	-	207	WP_000547939.1	hypothetical protein	-
ECTO6_RS23875	17994..18152	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	18196..18420	-	225	NuclAT_0	-	Antitoxin
-	18196..18420	-	225	NuclAT_0	-	Antitoxin
-	18196..18420	-	225	NuclAT_0	-	Antitoxin
-	18196..18420	-	225	NuclAT_0	-	Antitoxin
ECTO6_RS25050	18232..18420	+	189	WP_001299721.1	hypothetical protein	-
ECTO6_RS23880	18432..19151	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
ECTO6_RS23885	19148..19582	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
ECTO6_RS23890	19637..21595	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
ECTO6_RS23895	21654..21887	-	234	WP_000006004.1	DUF905 domain-containing protein	-
ECTO6_RS23900	21943..22470	-	528	WP_000290793.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / tet(A) / mph(B) / sul1 / qacE / ant(3'')-Ia / dfrA1 / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / catA1	iroN / iroE / iroD / iroC / iroB	1..174857	174857

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T292721 WP_001312861.1 NZ_LS992167:c18152-17994 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT292721 NZ_LS992167:c18420-18196 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin